Study on Salt Tolerance of AtUNE12 Gene in Arabidopsis thaliana

doi:10.7525/j.issn.1673-5102.2020.02.013

Abstract

Abstract: Members of the bHLH transcription factor family plays an important role in plant growth, metabolism and abiotic stress response. The salt tolerance of AtUNE12 gene that was part of stress-related bHLH transcription factor family from Arabidopsis thaliana was analyzed. Firstly, the plant overexpression vector of AtUNE12(pROKII-AtUNE12) was constructed, the transgenic T₃ generation A.thaliana was obtained by Agrobacterium-mediated dip then verified by qRT-PCR. Under salt stress, the growth, root length and fresh weight of overexpression and wild-type A.thaliana were analyzed. The MDA content, electrolyte leakage and water loss rate of overexpression and wild-type A.thaliana were compared. H₂O₂ content determination and SOD, POD activity was used to study overexpression and wild-type A.thaliana plants. The results showed that overexpression of AtUNE12 gene could significantly decrease MDA content, electrolyte leakage and the water loss rate, improved POD and SOD activity, and decreased H₂O₂ content in A.thaliana plants. The results indicated that AtUNE12 gene protected the integrity of cell membrane structure, and then enhanced the ROS scavenging ability of A.thaliana plants, thereby improving the salt tolerance of A.thaliana.

Key words: bHLH transcription factor, AtUNE12, Arabidopsis thaliana, salt tolerance, stress tolerance analysis

CLC Number:

Q943.2

LI Zi-Yi, HE Zi-Hang, LU Hui-Jun, WANG Yu-Cheng, JI Xiao-Yu. Study on Salt Tolerance of AtUNE12 Gene in Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2020, 40(2): 257-265.

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