Isolation and Identification of a Novel Enlarged Leaf Mutant atscamp in Arabidopsis thaliana

doi:10.7525/j.issn.1673-5102.2024.02.008

Abstract

Abstract:

Leaf is the main photosynthetic organ， and the leaf morphology beneficial for photosynthesis has become an important breeding target. atscamp was screened from the Arabidopsis thaliana mutant library（about 6 000 lines） with wide leaves. Tail-PCR analysis revealed a T-DNA insertion at the AT1G11180 locus， which encodes a secretory carrier membrane protein（SCAMP）. RT-PCR showed that the transcriptional expression level of the gene was basically zero. The leaf width and area of the mutant were significantly larger than those of wild type plants（P<0.01）， while the crown diameter was essentially unchanged. Additionally， chlorophyll content， chlorophyll maximium fluorescence， and photosystem II potential photochemical efficiency were all increased in the atscamp mutant（P<0.05）. Correspondingly， the mutants showed significant increases in transpiration coefficient（T_r）， net photosynthetic rate（P_n）， and leaf water use efficiency（W_UE）（P<0.05）. Spatial-temporal specific expression analysis of the AT1G11180 gene revealed that the gene was highly expressed only in leaves， with low expression levels in other organs， and the gene expression gradually increased with the development and maturity of plants. The results suggested that AtSCAMP might play an important role in leaf shape development.

Key words: Arabidopsis thaliana, AtSCAMP gene, leaf shape, mutant

CLC Number:

Q754

Xuefeng HAO, Xiaoyu JIA, Haiyan CAO, Chunxia KANG, Yanxi PEI. Isolation and Identification of a Novel Enlarged Leaf Mutant atscamp in Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2024, 44(2): 232-238.

Figures/Tables 7

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Fig.1

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引物 Primer	引物序列（5′→3′） Primer sequence（5′→3′）
AtSCAMP（F-RT-PCR）	ATCATCCCCTCGACGTACTGTAC
AtSCAMP（R-RT-PCR）	CTGGTTTTATATACAGCAGTCACG
AtSCAMP（F-qRT-PCR）	TCAGTGGTTAGCATCTGGGTT
AtSCAMP（R-qRT-PCR）	TGCCTCATATGGCAGCTCTC
AtACTIN（F-RT-PCR）	CTCAGCACCTTCCAACAGATGTGGA
AtACTIN（R-RT-PCR）	CCAAAAAAATGAACCAAGGACCAAA
AtACTIN（F-qRT-PCR）	TATGAATTACCCGATGGGCAAG
AtACTIN（R-qRT-PCR）	TGGAACAAGACTTCTGGGCAT
P1	CTACCTCTTTTCAACACCGA
P2	AGTAACGGCGATGATATTCC
P3	ATTTTGCCGATTTCGGAAC

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