Function of Populus trichocarpa HDA902 Gene in Response to Cold Stress

doi:10.7525/j.issn.1673-5102.2020.02.012

Abstract

Abstract: Histone deacetylases plays an important role in plant abiotic stress responses. Histone deacetylase gene HDA902 was cloned from Populus trichocarpa by RT-PCR. The HDA902 gene was transformed into tobacco by Agrobacterium tumefaciens-mediated method. The tolerance of transgenic plants to cold stress was analyzed. The results showed that the expression of HDA902 in tobacco significantly improved the tolerance of transgenic lines to low temperature. The results of NBT and DAB staining showed that transgenic tobacco produced less reactive oxygen species than the wild type after cold treatment. The proline content in the leaves of transgenic plants were much higher than that of wild-type plants, whereas the MDA contents in the leaves of transgenic plants were much lower at low temperature. These results indicated that HDA902 was involved in the response to cold stress, and its overexpression enhanced the tolerance of transgenic plants to cold stress.

Key words: tobacco, HDA902, cold stress, tolerance

CLC Number:

S722.3+6

GUAN Tao, LIU Chao, LI Kai-Long, XIA De-An, MA Xu-Jun. Function of Populus trichocarpa HDA902 Gene in Response to Cold Stress[J]. Bulletin of Botanical Research, 2020, 40(2): 251-256.

References 21

[1]	Jung J H,Park J H,Lee S,et al.The cold signaling attenuator HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 activates FLOWERING LOCUS C transcription via chromatin remodeling under short-term cold stress in Arabidopsis[J].The Plant Cell,2013,25(11):4378-4390.
[2]	陈儒钢,巩振辉,逯明辉,等.植物抗寒基因工程研究进展[J].西北植物学报,2008,28(6):1274-1280.Chen R G,Gong Z H,Lu M H,et al.Research advance in the cold-resistance genetic engineering in plants[J].Acta Botanica Boreali-Occidentalia Sinica,2008,28(6):1274-1280.
[3]	陈霞,李宜珅,仇奕之,等.低温胁迫对转高山离子芥CbPLDα、CbPLDβ基因烟草抗氧化酶系统的影响[J].中国农学通报,2015,31(34):209-215.Chen X,Li Y K,Qiu Y Z,et al.Effect of low temperature stress on antioxidant system of CbPLDα and CbPLDβ transgenic tobaccos[J].Chinese Agricultural Science Bulletin,2015,31(34):209-215.
[4]	郭惠明,李召春,张晗,等.棉花CBF基因的克隆及其转基因烟草的抗寒性分析[J].作物学报,2011,37(2):286-293.Guo H M,Li Z C,Zhang H,et al.Cloning of cotton CBF gene and its cold tolerance expression in transgenic tobacco[J].Acta Agronomica Sinica,2011,37(2):286-293.
[5]	Hollender C,Liu Z C.Histone deacetylase genes in Arabidopsis development[J].Journal of Integrative Plant Biology,2008,50(7):875-885.
[6]	Hu Y F,Qin F J,Huang L M,et al.Rice histone deacetylase genes display specific expression patterns and developmental functions[J].Biochemical and Biophysical Research Communications,2009,388(2):266-271.
[7]	Rossi V,Locatelli S,Varotto S,et al.Maize histone deacetylase hda101 is involved in plant development,gene transcription,and sequence-specific modulation of histone modification of genes and repeats[J].The Plant Cell,2007,19(4):1145-1162.
[8]	Chen L T,Luo M,Wang Y Y,et al.Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response[J].Journal of Experimental Botany,2010,61(12):3345-3353.
[9]	To T K,Nakaminami K,Kim J M,et al.Arabidopsis HDA6 is required for freezing tolerance[J].Biochemical and Biophysical Research Communications,2011,406(3):414-419.
[10]	Zhou C H,Zhang L,Duan J,et al.HISTONE DEACETYLASE19 is involved in jasmonic acid and ethylene signaling of pathogen response in Arabidopsis[J].The Plant Cell,2005,17(4):1196-1204.
[11]	Kim K C,Lai Z B,Fan B F,et al.Arabidopsis WRKY38 and WRKY62 transcription factors interact with histone deacetylase 19 in basal defense[J].The Plant Cell,2008,20(9):2357-2371.
[12]	刘超,李开隆,刘春娟,等.毛果杨HDA902基因的克隆、序列分析和亚细胞定位[J].分子植物育种,2016,14(5):1082-1090.Liu C,Li K L,Liu C J,et al.Cloning,sequence analysis and subcellular localization of HDA902 gene from Populus trichocarpa[J].Molecular Plant Breeding,2016,14(5):1082-1090.
[13]	Ma X J,Lü S B,Zhang C,et al.Histone deacetylases and their functions in plants[J].Plant Cell Reports,2013,32(4):465-478.
[14]	Chen L T,Wu K Q.Role of histone deacetylases HDA6 and HDA19 in ABA and abiotic stress response[J].Plant Signaling & Behavior,2010,5(10):1318-1320.
[15]	Luo M,Wang Y Y,Liu X C,et al.HD2C interacts with HDA6 and is involved in ABA and salt stress response in Arabidopsis[J].Journal of Experimental Botany,2012,63(8):3297-3306.
[16]	Alinsug M V,Yu C W,Wu K Q.Phylogenetic analysis,subcellular localization,and expression patterns of RPD3/HDA1 family histone deacetylases in plants[J].BMC Plant Biology,2009,9:37.
[17]	Fu W Q,Wu K Q,Duan J.Sequence and expression analysis of histone deacetylases in rice[J].Biochemical and Biophysical Research Communications,2007,356(4):843-850.
[18]	Zheng Y,Ding Y,Sun X,et al.Histone deacetylase HDA9 negatively regulates salt and drought stress responsiveness in Arabidopsis[J].Journal of Experimental Botany,2016,67(6):1703-1713.
[19]	Ma X J,Zhang B,Liu C J,et al.Expression of a populus histone deacetylase gene 84KHDA903 in tobacco enhances drought tolerance[J].Plant Science,2017,265:1-11.
[20]	Hu Y,Zhang L,Zhao L,et al.Trichostatin A selectively suppresses the cold-induced transcription of the ZmDREB1 gene in maize[J].PLoS One,2011,6(7):e22132.
[21]	董亚茹.毛果杨HDAC基因家族与低温胁迫反应关系的初步研究[D].哈尔滨:东北林业大学,2013.Dong Y R.Involvment of the Populus trichocarpa HDAC gene family in the cold stress response[D].Harbin:Northeast Forestry University,2013.