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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (2): 274-283.doi: 10.7525/j.issn.1673-5102.2020.02.015

• Research Report • Previous Articles     Next Articles

Cloning of LpPEX7 Gene from Lilium pumilum and Its Expression Characteristics under Salt Stress

HE Hao, ZHU Guo-Qing, CHEN Shi-Ya, XU Chang, JIN Shu-Mei   

  1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040
  • Received:2019-10-10 Online:2020-03-05 Published:2020-03-06
  • Supported by:
    Nature Scientific Foundation of Heilongjiang Province project(LH2019C011);Open Fund Key of Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field(SAVER1701);National Natural Science Foundation of China(31070616,31500317)

Abstract: The peroxidasome biosynthetic gene(LpPEX7) of Lilium pumilum DC was cloned, and its open reading frame length is 957 bp, encoding 318 amino acids. The deduced amino acid sequence of LpPEX7 contains six WD40 conserved domains, through sequence alignment analysis of homologous proteins and evolutionary tree analysis, it was found that LpPEX7 had higher homology with some PEX7 from other plants. The expression of LpPEX7 was higher in seeds, leaves and bulbs, but lower in roots and flowers of L.pumilum. The expression of LpPEX7 changed under H2O2, NaCl and NaHCO3 stress conditions. Subsequently, the seeds germination of LpPEX7 over-expression Arabidopsis thaliana lines as earlier than that of wild seeds under the treatment of salinity and oxidative stress. These results show that the LpPEX7 gene has a certain response relationship with salinity and oxidative adversity. It provides a very important candidate gene for the study of salt-tolerant alkaline molecular mechanism of Lily.

Key words: Lilium pumilum DC, peroxidasome biosynthetic gene, gene expression, Arabidopsis thaliana

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