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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (3): 407-415.doi: 10.7525/j.issn.1673-5102.2020.03.012

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Resistance Analysis of Transgenic PnDof30 Arabidopsis under Abiotic Stress

ZHANG Yu-Qing1, LIU Ye1, QU Chun-Pu2, LIU Guan-Jun2, YANG Tian-Tian3, YANG Cheng-Jun1   

  1. 1. College of Forestry, Northest Forestry University, Harbin 150040;
    2. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
    3. College of Wildlife and Nature Conservation, Northeast Forestry University, Harbin 150040
  • Received:2019-10-17 Online:2020-05-05 Published:2020-05-29
  • Supported by:
    This work was supported by the Fundamental Research Funds for the Central Universities(2572017CA07);Postdoctoral Scientific Research Developmental Fund of Heilongjiang Province(LBH-Q15005)

Abstract: Dof(DNA-binding with one finger) transcription factor is a unique transcription factor in plants,it is a family of zinc finger proteins with many members,the length of amino acids is generally between 200-400, and it contains very conservative N-terminal and relatively changeable C-terminal. Previous studies have shown that Dof transcription factor family plays an important role in regulating carbon and nitrogen metabolism, improving nitrogen uptake and utilization, and enhancing abiotic stress tolerance. To explore the resistance of PnDof30 gene in Populus simonii×P.nigra, this study used transgenic PnDof30 Arabidopsis thaliana as a research object to compare the physiological and biochemical indices of over-expressed PnDof30 Arabidopsis line L2 and wild type A.thaliana under drought, salt and osmotic stress. It was found that the seed germination rate, root length and fresh weight of L2 were higher than those of WT; the contents of SOD, POD and proline were higher than those of WT, while the contents of chlorophyll and MDA were decreased; the PnDof30 gene in L2 was significantly increased after stress. These results indicate that PnDof30 gene has the ability to resist drought, salt and osmotic stress. The results of this experiment will be of great significance for understanding the stress resistance of Dof transcription factors.

Key words: Populus simonii×P.nigra, Dof transcription factor, transgenic PnDof30 Arabidopsis thaliana, abiotic stress

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