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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 531-539.doi: 10.7525/j.issn.1673-5102.2021.04.008

• Research report • Previous Articles    

Structural and Functional Analysis of Gibberellin Signaling Transduction Pathway Gene HbRGA1 in Rubber Tree

Hua-Feng CHEN1, Song-Le FAN2, Li-Feng WANG2()   

  1. 1.College of Chemistry,Chemical Engineering and Resource Utilization,Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
    2.Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,P. R. China;State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops;Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,P. R. China;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
  • Received:2020-12-15 Online:2021-07-20 Published:2021-03-24
  • Contact: Li-Feng WANG E-mail:lfwang@catas.cn
  • About author:CHEN Hua-Feng(1978—),male,postgraduate,major in plant Metabolism and Physiological Ecology.
  • Supported by:
    National Key Research and Development Project(2020YFD1001203)

Abstract:

DELLA protein is a negative regulator of gibberellin signaling pathway, which inhibits plant growth and development. Analyzing the structure and function of DELLA family members will be helpful to elucidate the mechanism by which the DELLA protein regulates the growth and development of rubber trees. In this study, the full-length cDNA sequence of HbRGA1 was cloned from the leaves of rubber tree cultivar Reyan73397. HbRGA1 is 2 136 bp long, contains 1 839 bp ORF, and encodes 613 amino acids. The HbRGA1 protein sequence contains DELLA and GRAS superfamily conserved domains, which are 82.5% like the DELLA genes of poplar, cassava and rubber trees. qRT-PCR analysis showed that the expression level of HbRGA1 was high in the leaf of rubber tree, but very low in the bark and latex. The expression of HbRGA1 in leaves was significantly up-regulated by gibberellin and abscisic acid spraying. These results indicated that HbRGA1 was closely related to gibberellin and other hormone signals in rubber trees, which laid a good foundation for further study of HbRGA1’s structure and function in the growth and development of rubber trees.

Key words: Hevea brasiliensis, HbRGA1, gibberellin, gene expression

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