Sequencing Analysis of Transcriptome of Male Floral Bud at Two Development Stages in Eucommia ulmoides

doi:10.7525/j.issn.1673-5102.2020.02.016

Abstract

Abstract: The male floral bud Eucommia ulmoides is rich in healthily kinds of nutrition compositions and active matter, which with a high nutritional and medicinal value. The transcriptome of male floral bud during two differentiation stages was sequenced, aimed to comprehend the expression of genes involved in the stamen development of E.ulcommia. lllumina high-throughput sequencing technology was applied to detect the transcriptome sequencing data of the male flora bud of ‘Huazhong No.11’ during bract differentiation and stamen differentiation stages. Obtained clean data was analyzed including gene function annotation and differentially expressed genes(DEGs) screening was analyzed by bioinformatics methods. A total of 40.48 Gb clean data were obtained, approximately 90.00% of the clean reads were mapped to the E.ulmoides reference genome. It was found that 315 genes were significantly up-regulated and 269 genes were significantly down-regulated in the male flower buds at stamen differentiation stage. GO and KEGG analyses of DEGs showed the DEGs were enriched in development process, photoperiodism, hormone biosynthetic and signal transduction process, carbon metabolism and other processes and pathways associated with flower induction. The photoperiodic was perhaps the most important pathway for the floral induction of E.ulcommia, and the carbohydrates, plant hormone and other metabolic substances was required in the process of male floral bud differentiation. Importantly, the members of MADs-box including FLC, SOC1, AGL3 and AGL8 regulated the stamen formation of E.ulcommia. It provides a significant reference for studying the genes involved in the floral organs and molecular breeding of E.ulcommia.

Key words: Eucommia ulmoides, male floral bud, different developmental stages, transcriptome, differentially expressed genes

CLC Number:

S722.3

ZHU Li-Li, DU Qing-Xin, HE Feng, QING Jun, DU Hong-Yan. Sequencing Analysis of Transcriptome of Male Floral Bud at Two Development Stages in Eucommia ulmoides[J]. Bulletin of Botanical Research, 2020, 40(2): 284-292.

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