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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (4): 484-487.doi: 10.7525/j.issn.1673-5102.2012.04.018

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In vitro Culture of Endangered Medicinal Plant Sinopodophyllum hexandrum Royle

GUO Qi;ZHANG Jun;ZHAO Xiao-Feng;YANG Tao;WANG Pei-Ya;YANG Hui;*   

  1. 1.Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000;2.Industrial Microbiology Engineering Research Center in Gansu,Lanzhou 730000
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-20 Published:2012-07-20
  • Contact: YANG Hui
  • Supported by:

Abstract: The seeds of Sinopodophyllum hexandrum Royle were taken as materials. The effects of exogenous hormones on induction, multiplication and rooting of explants were discussed. The regeneration system of S.hexandrum has been established. The results showed that: The optimal conditions for shoot induction were 3.0 mg·L-1 6-BA+0.5 mg·L-1 GA-3. The induction rate was 85.71% with a shortening of 30-40 d. For multiplication, the optimal conditions were 3.0 mg·L-1 6-BA+0.2 mg·L-1 IAA, and the mean shoots obtained per explant was 1.63. The most proper rooting medium was: WPM+1.5 mg·L-1 IAA+0.5 mg·L-1 NAA and the rooting ratio was 60.1% after 30 d culturing.

Key words: Sinopodophyllum hexandrum Royle, seeding, hormones, In vitro culture

CLC Number: