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植物研究 ›› 2011, Vol. 31 ›› Issue (4): 495-498.doi: 10.7525/j.issn.1673-5102.2011.04.018

• 论文 • 上一篇    下一篇

大连地区岛屿与大陆藜(Chenopodium album L.)种群遗传多样性的ISSR分析

张恒庆;田菲菲;贾俊玲;谭琨;杜洋;房孝迪;吴锦美   

  1. 辽宁师范大学生命科学学院,大连 116029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-07-20 发布日期:2011-07-20
  • 基金资助:
     

Analysis of Genetic Diversity between Island and Mainland Natural Populations of Chenopodium album L. in Dalian Area by ISSR

ZHANG Heng-Qing;TIAN Fei-Fei;JIA Jun-Ling;TAN Kun;DU Yang;FANG Xiao-Di;WU Jin-Mei   

  1. College of Life Sciences,Liaoning Normal University,Dalian 116029
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20
  • Supported by:
     

摘要: 采用ISSR分子标记对分布于大连市周边的4个藜的天然种群100个个体进行了遗传多样性及遗传结构的研究。13个引物共检测了157个位点,多态位点比率为67.52%,Shannon信息指数在物种水平上为0.332 9。根据GST值,藜遗传变异有40.03%发生在种群间。遗传距离分析表明,DT种群与MLN种群遗传一致度最高,遗传距离与地理距离之间没有相关性。

关键词: 藜, 遗传多样性, ISSR, 遗传结构

Abstract: Using ISSR method, the genetic diversity and genetic structure of 100 individuals in four natural populations of Chenopodium album L. distributed in Dalian surrounding were studied in this paper. Through the amplification of ISSR with 13 primers, 157 loci were detected and the percentage of polymorphic loci was 67.52. Shannon’s information index was 0.332 9 at the species level, genetic variance was 40.03% among populations according to GST value. The genetic distances analysis showed that DT population and MLN population have the highest population genetic identity, genetic distances and geographic distances have no correlation.

Key words: Chenppodium album L, ISSR, genetic diversity, genetic structure

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