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植物研究 ›› 2020, Vol. 40 ›› Issue (6): 923-931.doi: 10.7525/j.issn.1673-5102.2020.06.015

• 研究报告 • 上一篇    

基于SSR技术对黄河三角洲二色补血草遗传多样性研究

吕冬雪, 张学杰, 张洛艳, 樊守金()   

  1. 山东师范大学生命科学学院逆境植物重点实验室,济南 250014
  • 收稿日期:2019-12-15 出版日期:2020-11-20 发布日期:2020-11-04
  • 通讯作者: 张洛艳,樊守金 E-mail:fanshoujin007@ 163.com;fanshoujin007@163.com
  • 作者简介:吕冬雪(1994—),女,硕士研究生,主要从事植物资源分类研究。
  • 基金资助:
    全国中药资源普查项目财社[2018]43号

Analysis of Limonium bicolor by SSRMolecular Markers in the Yellow River Delta Region

Dong-Xue LÜ, Xue-Jie ZHANG, Luo-Yan ZHANG, Shou-Jin FAN()   

  1. Key Laboratory of Plant Stress Research,College of Life Science,Shandong Normal University,Jinan 250014
  • Received:2019-12-15 Online:2020-11-20 Published:2020-11-04
  • Contact: Luo-Yan ZHANG,Shou-Jin FAN E-mail:fanshoujin007@ 163.com;fanshoujin007@163.com
  • About author:LÜ Dong-Xue(1994—),female,postgraduate,mainly engaged in classification of plant resources research.
  • Supported by:
    National Chinese Medicine Resource Census Project Finance[2018](43)

摘要:

应用GENALEX6.502、ARLEQUINversion3.5、STRUCTUREv2.3.4、STRUCTURE Harvester、CLUMP和Distruct等软件进行遗传参数估算、主成分分析、遗传变异分析及遗传结构分析。在这项研究中,我们系统地从分布在中国黄河三角洲的12个二色补血草居群中采集到202份个体。我们通过使用共显性的微卫星标记探索微地理遗传结构,旨在解决黄河三角洲二色补血草居群结构和动态问题。结果表明:二色补血草呈中等程度的遗传分化,遗传变异主要存在于居群内,微弱的遗传分化存在于居群间,为(FST=0.067)。香农信息指数平均是1.037,基因流平均是4.106,观测杂合度和期望杂合度平均值分别是0.43和0.529。通过分析二色补血草群体的遗传多样性和遗传结构,旨在为资源的管理、保护和利用提供依据。总之,保护遗传多样性时,应保存尽可能多的居群和特异单株。

关键词: 二色补血草, 遗传多样性, SSR, 土壤盐度, 毛细管电泳

Abstract:

Genetic diversity parameters, principal coordinates analysis(PCoA), and analysis of molecular variance(AMOVA) were conducted by using GENALEX6.502 and ARLEQUINv3.5 software. STRUCTUREv 2.3.4, STRUCTURE Harvester, CLUMP, and Distruct software were used to analyze genetic structure. We systematically sampled 202 individuals from 12 Limonium bicolor populations distributed throughout the Yellow River Delta(YRD) region in China. By exploring the micro-geographic genetic structure using the codominant microsatellite(SSR) markers, we aimed to answer questions as to what extent the population structure and dynamics of L.bicolor YRD. The demonstrated a medium level of genetic differentiation in L.bicolor. The genetic variation was mainly maintained within populations, with a weak but significant genetic differentiation being detected among populations(FST=0.067). Shannon’s information index was 1.037, and gene flow was 4.106, the observed heterozygosity(Ho) and expected heterozygosity(He) was 0.43 and 0.529. Genetic diversity and population genetic structure were analyzed in L.bicolor to provide useful information for management, protection and utilization. In brief, suggesting that more individuals from different populations should be collected to protect the genetic diversity of L.bicolor.

Key words: Limonium bicolor, genetic diversity, SSR, soil salinity, capillary electrophoresis

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