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植物研究 ›› 2020, Vol. 40 ›› Issue (4): 623-628.doi: 10.7525/j.issn.1673-5102.2020.04.017

• 研究简报 • 上一篇    

白藜芦醇脂质载体制备及体外抗氧化活性测定

王欣宇1,2,3, 李弘琨1,2,3, 于良1,2,3, 赵雪莲1,2,3, 于雪莹1,2,3, 付玉杰1,2,3   

  1. 1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
    2. 东北林业大学林业生物制剂教育部工程研究中心, 哈尔滨 150040;
    3. 东北林业大学化学化工与资源利用学院, 哈尔滨 150040
  • 收稿日期:2020-03-07 发布日期:2020-06-12
  • 通讯作者: 付玉杰,E-mail:yujie-fu@163.com E-mail:yujie-fu@163.com
  • 作者简介:王欣宇(1995-),女,硕士研究生,主要从事植物学方面的研究。
  • 基金资助:
    “十三五”国家重点研发计划项目(2016YFD0600805);黑龙江省应用技术研究与开发项目(GX18B003);111创新引智项目(B20088)

Preparation of Resveratrol-Nanostructured Lipid Carrier and Evaluation of in vitro Antioxidant Activity

WANG Xin-Yu1,2,3, LI Hong-Kun1,2,3, YU Liang1,2,3, ZHAO Xue-Lian1,2,3, YU Xue-Ying1,2,3, FU Yu-Jie1,2,3   

  1. 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040;
    3. College of Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040
  • Received:2020-03-07 Published:2020-06-12
  • Supported by:
    National Key R&D Program of China(2016YFD0600805);Heilongjiang Province Applied Technology Research and Development Project(GX18B003);Innovative and intelligent Projects 111(B20088)

摘要: 白藜芦醇是一种天然功能性成分,具有美白、抗氧化等功效,可用于治疗改善多种皮肤病变,延缓细胞衰老。白黎芦醇在水中的溶解度低,稳定性较差,影响了其在医药及化妆品领域的应用。本研究制备了负载白黎芦醇的纳米结构脂质载体,提高了白藜芦醇的水中分散度、稳定性和功能活性。通过热高压均质法制备了白黎芦醇纳米结构脂质载体(NLC),确定了以单硬脂酸甘油酯为固态脂质,油酸为液态脂质,其比例为1:1,4%吐温80为乳化剂,均质压力为600 bar,循环3次时得到的白藜芦醇脂质载体粒径为179 nm,此时药物包封率为85.33%。MTT实验和ROS清除实验结果也表明白藜芦醇脂质载体浓度低于100 μmol·L-1时都未对细胞产生明显毒性,并且有优于白藜芦醇原料药的抗氧化活性。

关键词: 白藜芦醇, 纳米脂质载体, 热高压均质, 粒径, 抗氧化

Abstract: Resveratrol is a natural functional component, with whitening, antioxidant and other effects, can be used to improve skin lesions and cell aging. Resveratrol has low solubility and poor stability in water, which impedes its application in medicine and cosmetics. The nanostructured lipid carrier loaded with resveratrol was prepared to improve the stability and functional activity of resveratrol. The resveratrol nanostructured lipid carrier(NLC) was prepared by thermal high pressure homogenization method, and the homogenization pressure was determined to be 600 bar with GMS as the solid lipid, oleic acid as the liquid lipid, with their ratio of 1:1, 4% Tween 80 as the emulsifier, and the smallest particle size of resveratrol lipid carrier was 179 nm after three cycles.The results of MTT experiment and ROS scavenging experiment also showed that resveratrol did not produce obvious toxicity to cells when the lipid carrier concentration of resveratrol was less than 100 μmol·L-1, and had better antioxidant activity than resveratrol API.

Key words: resveratrol, nanostructure lipid carrier, hot high-pressure homogenization(HPH), particle size, antioxidant

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