22种木莲属植物亲缘关系的ISSR分析

doi:10.7525/j.issn.1673-5102.2011.04.017

植物研究 ›› 2011, Vol. 31 ›› Issue (4): 489-494.doi: 10.7525/j.issn.1673-5102.2011.04.017

22种木莲属植物亲缘关系的ISSR分析

肖黎¹；李晓玲¹；王玉兵¹；王希群²；陈发菊^1*

1.三峡大学生物技术研究中心,宜昌　443002；2.国家林业局林产工业规划设计院,北京　100010

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-07-20 发布日期:2011-07-20
通讯作者: 陈发菊
基金资助:

Analyses of the Genetic Relationships Among 22 Species of Manglietia Plants Using ISSR Markers

XIAO Li;LI Xiao-Ling;WANG Yu-Bing;WANG Xi-Qun;CHEN Fa-Ju*

1.Biotechnology Research Center,China Three Gorges University,Yichang　443002；2.Planning and Design Academy of Forest Products Industry,State Forestry Administration,Beijing　100010

Received:1900-01-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20
Contact: CHEN Fa-Ju
Supported by:

摘要/Abstract

摘要： 利用ISSR标记分析技术探讨了22种木莲属植物的遗传亲缘关系。选用10对ISSR引物组合对木莲属22个种的基因组进行分析。结果表明：22个种共扩增出595条DNA带，其中多态性条带占94.45%；根据NeiLi遗传相似性系数在0.68处，UPGMA聚类结果将22个种划分为6个类群，在0.70处进一步可分为12个亚类群；22种木莲属植物的Nei-Li遗传相似性系数变化范围为0.605(乳源木莲与球果木莲之间)～0.956(中缅木莲与滇南木莲之间)，平均遗传相似性系数为0.698。说明木莲属植物种间差异性大。其中中缅木莲、滇南木莲之间遗传相似性系数为0.956，两者亲缘关系最近。该结果同时支持将巴东木莲、乳源木莲、滇桂木莲分别作为独立的种。

关键词: 木莲属植物, ISSR分析, 亲缘关系

Abstract: The genetic relationships among 22 species of Manglietia plants were analyzed by using inter-simple sequence repeat（ISSR) molecular-marked technique. Ten ISSR primers were selected to assess the genomes of 22 species of Manglietia plants. The result showed that a total of 595 DNA bands were amplified and 562 of which (94.45%) were polymorphic. According to the Nei-Li genetic similarity of 0.68, UPGMA method cluster analysis indicated that these 22 species were classified into 6 cluster groups, and they were classified into 12 sub-cluster groups with the genetic similarity of 0.70. The Nei-Li genetic similarities of 22 species of Manglietia ranged from 0.605 (between M.yuyuanensis and M.conifera) to 0.956 (between M.hookeri and M.wangii). The average genetic similarity is 0.698, which suggests that Manglietia have a plenty of inter-species diversity. The genetic similarity coefficient between M.hookeri and M.wangii is 0.956, and their genetic relationship is actually the closest. The result supports the classification of M.patungensis, M.yuyuanensis and M.forrestii as independent species respectively.

Key words: Manglietia, ISSR analysis, genetic relationships

中图分类号:

S792

肖黎;李晓玲;王玉兵;王希群;陈发菊*. 22种木莲属植物亲缘关系的ISSR分析[J]. 植物研究, 2011, 31(4): 489-494.

XIAO Li;LI Xiao-Ling;WANG Yu-Bing;WANG Xi-Qun;CHEN Fa-Ju*. Analyses of the Genetic Relationships Among 22 Species of Manglietia Plants Using ISSR Markers[J]. Bulletin of Botanical Research, 2011, 31(4): 489-494.

[1]	过雪莹, 朱凯琳, 陈昕, 付雪晴, 唐东芹. 6个香雪兰品种的染色体核型及聚类分析[J]. 植物研究, 2022, 42(4): 637-646.
[2]	杨斌, 孟庆瑶, 张凯, 段义忠. 孑遗濒危植物矮扁桃叶绿体全基因组特征分析及亲缘关系鉴定[J]. 植物研究, 2020, 40(5): 686-695.
[3]	孙忆, 丁苏芹, 史益敏, 唐东芹. 15个小苍兰品种的花粉形态研究[J]. 植物研究, 2019, 39(1): 17-26.
[4]	丑欢欢, 唐红. 基于ITS和matK基因对牡丹组序列分析及其亲缘关系的研究[J]. 植物研究, 2017, 37(4): 603-612.
[5]	林立；王志龙*；付涛；林乐静. 39个樱花品种亲缘关系的ISSR分析[J]. 植物研究, 2016, 36(2): 297-304.
[6]	傅建敏1,2；梁晋军1,2；乌云塔娜1,2；梁玉琴1,2；孙鹏1,2；李芳东1,2. 基于叶绿体DNA ndh*A和nrDNA ITS序列变异分析栽培柿及其近缘种亲缘关系[J]. 植物研究, 2015, 35(4): 515-520.
[7]	饶龙兵1；杨汉波1；郭洪英2；段红平3；陈益泰1. 17种桤木属植物的亲缘关系研究及模糊种鉴定[J]. 植物研究, 2015, 35(4): 528-534.
[8]	李晓玲；杨进；张雄；王雪松. 10份续断属植物亲缘关系的ISSR分析[J]. 植物研究, 2015, 35(3): 450-456.
[9]	胡仲义;林立;刘夔;曾传圣. 26个川茶花品种亲缘关系的ISSR分析[J]. 植物研究, 2013, 33(5): 629-634.
[10]	陈燕英;黎云祥*;权秋梅;廖兴利;成仙鸽. 7种川产淫羊藿属植物遗传多样性及亲缘关系的ISSR分析[J]. 植物研究, 2012, 32(2): 208-212.
[11]	杨帆;曾丽*;叶康;赵子刚;张嫔;龚霄雯;尹勤;孙强. 17份蔷薇属植物的亲缘关系的形态学和ISSR分析[J]. 植物研究, 2011, 31(2): 193-198.
[12]	姚庆荣;赵长增;王文泉*. 海南部分荔枝种质资源亲缘关系的SSR分析[J]. 植物研究, 2009, 29(5): 628-632.
[13]	贺随超;马履一*. 红花玉兰与玉兰亚属几个种亲缘关系的AFLP分析[J]. 植物研究, 2008, 28(3): 288-292.
[14]	王铁娟;韩国栋. 中国蒿属沙生地理替代种亲缘关系的RAPD分析[J]. 植物研究, 2007, 27(4): 449-454.
[15]	严一字;吴基日*. 利用RAPD标记分析东亚地区桔梗的亲缘关系[J]. 植物研究, 2007, 27(3): 308-312.

22种木莲属植物亲缘关系的ISSR分析

Analyses of the Genetic Relationships Among 22 Species of Manglietia Plants Using ISSR Markers

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价