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植物研究 ›› 2022, Vol. 42 ›› Issue (5): 780-789.doi: 10.7525/j.issn.1673-5102.2022.05.009

• 遗传与育种 • 上一篇    下一篇

rpl32-trnL片段在柳穿鱼不同地理居群遗传多样性分析中的应用

张玉洁, 石鹏霞, 赵凡, 陈晓丹, 孙坤, 张辉()   

  1. 西北师范大学生命科学学院,兰州 730070
  • 收稿日期:2022-03-29 出版日期:2022-09-20 发布日期:2022-09-15
  • 通讯作者: 张辉 E-mail:zhanghui@nwnu.edu.cn
  • 作者简介:张玉洁(1997—),女,硕士研究生,主要从事系统与进化植物学方向的研究。
  • 基金资助:
    国家自然科学基金项目(31060033);中科院遗传发育所染色体工程国家重点实验室开放基金项目(PCCE-KF-2019-06)

Genetic Diversity Analysis from Different Geographic Populations of Linaria vulgaris var. sinensis by rpl32-trnL Fragment

Yujie ZHANG, Pengxia SHI, Fan ZHAO, Xiaodan CHEN, Kun SUN, Hui ZHANG()   

  1. College of Life Science,Northwest Normal University,Lanzhou 730070
  • Received:2022-03-29 Online:2022-09-20 Published:2022-09-15
  • Contact: Hui ZHANG E-mail:zhanghui@nwnu.edu.cn
  • About author:ZHANG Yujie(1997—),female,master,major in plant systematics and evolution.
  • Supported by:
    Under the auspices of the National Natural Science Foundation of China(31060033);Open funded Fund of the State Key Laboratory for Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences(PCCE-KF-2019-06)

摘要:

为了探究柳穿鱼(Linaria vulgaris)不同地理居群的遗传多样性,利用叶绿体DNA的rpl32-trnL片段对包含62个个体的4个柳穿鱼地理居群遗传多样性进行了研究。结果显示:柳穿鱼4个居群中共检测到15种单倍型和76个变异位点,总遗传多样性较高(Hd=0.878,π=0.003 88,K=2.994),遗传变异主要存在于居群内(51.49%),隶属于柳穿鱼虫媒异交繁殖策略的遗传特征;不同地区柳穿鱼居群间遗传分化大(0.466 14),居群间基因交流水平较低(0.29);遗传分化程度与地理距离存在中等程度相关性但不显著(R2 = 0.36,P > 0.05)。中性检验显示除合水居群(HS)在进化过程中经历过瓶颈效应(Fu and Li’s D=-2.450 49,P<0.05)外,其他居群进化过程符合分子进化的中性理论。本研究结果不仅揭示了繁殖策略、地理隔离及生境干扰等因素塑造我国北方柳穿鱼居群遗传多样性和遗传结构特征,而且也为今后柳穿鱼资源保护策略的选择提供了理论依据。

关键词: 柳穿鱼, 地理居群, 遗传多样性, rpl32-trnL

Abstract:

In order to investigate the population genetic characteristics of Linaria vulgaris, four different geographic populations containing 62 individuals were analyzed by using rpl32-trnL fragment of chloroplast DNA. The results showed that there were 15 haplotypes and 76 loci existing in 4 populations. The total genetic diversity of L. vulgaris reached a high level(Hd=0.878, π=0.003 88, K=2.994). The AMOVA analysis showed that the genetic variations were mainly maintained within the populations(51.49%), which was consistent with the genetic characteristics of self-incompatibility in L. vulgaris. The genetic differentiation among four geographic populations was significant(FST=0.466 14), while the gene flow between different geographic populations was rather low(Nm=0.29). The genetic differentiation between different populations was positively correlated with the geographical distance but no significance(R2=0.36, P>0.05). The results of neutrality test showed that the Heshui population(HS) might experience some bottleneck events during its evolution (Fu and Li’s D=-2.450 49,P<0.05), and the evolutionary feature of the other populations was consistent with the neutral hypothesis. This study revealed that the reproductive strategies, geographic isolation, and habitat disturbance were not only responsible for the rather high level of genetic diversity in the populations distributed in the northern area of China as well as their genetic structure characteristics, but also it might provide a scientific basis for the further study of conservation genetics in L. vulgaris.

Key words: Linaria vulgaris, geographic populations, genetic diversity, rpl32-trnL

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