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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (1): 69-75.doi: 10.7525/j.issn.1673-5102.2023.01.008

• Physiology and Ecology • Previous Articles     Next Articles

Effects of Tapping Panel Dryness on Mitochondrial Ultrastructure and ROS Metabolism in Barks of Rubber Tree (Hevea brasiliensis)

Hong YANG1,2,3,4(), Lifeng WANG1,2,3,4, Longjun DAI1,2,3,4, Bingbing GUO1,2,3,4   

  1. 1.Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
    2.Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,Haikou 571101
    3.State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops,Haikou 571101
    4.Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,Haikou 571101
  • Received:2021-03-03 Online:2023-01-20 Published:2022-12-23
  • Contact: Hong YANG E-mail:yang_hong0317@126.com
  • About author:YANG Hong(1988—),male,postgraduate,assistant researcher,mainly engaged in physiological and molecular biology of rubber tree. E-mail:yang_hong0317@126.com
  • Supported by:
    Natural Science Foundation of Hainan Province(320QN350);Major Science and Technology Plan of Hainan Province(ZDKJ2021004);Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630022020015)


In order to elucidate the mechanism of tapping panel dryness(TPD) and effectively control it, the ultrastructure and the expression patterns of reactive oxygen species(ROS)-related genes in barks from healthy(H) and different degrees of TPD trees were studied in this study. The results showed that with the development of TPD, aberrant mitochondrial ultrastructure changed including the loss of the matrix, swelling of cristae, and the cavitation of the inner cavity were observed in barks. The results of qRT-PCR experiments showed that the expression levels of HbPOD2 and HbPOD3 in TPD trees were higher than those in healthy trees, indicating the possibility of these genes being used as “marker” genes to monitor the tapping intensity, the stimulation intensity, and the occurrence of TPD. The gene HbCAT was also down-regulated in TPD trees, indicating that the balance between ROS generation and scavenging may be a key factor in the occurrence of TPD in rubber tree. The expressions of HbGST1HbGST2, and HbPPO in TPD trees were higher than those in healthy trees, indicating these genes may be associated with in-situ coagulation of latex during TPD occurrence. This study reveals the changes of ultrastructure and the expression patterns of ROS-related genes in rubber tree barks during TPD occurrence. These findings may provide a new perspective for elucidating the mechanism of TPD in rubber tree, and provid theoretical guidance for developing methods for monitoring tapping intensity and stimulation intensity and for developing TPD-related “marker” genes in rubber trees.

Key words: Hevea brasiliensis, tapping panel dryness, ultrastructure, reactive oxygen species, gene expression

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