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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (3): 455-465.doi: 10.7525/j.issn.1673-5102.2022.03.015

• Physiology and Ecology • Previous Articles     Next Articles

Effects of Spraying NaHS at Different Growth Stages on H2S Production and Reactive Oxygen Species Metabolism of Naked Oat Leaves under Saline-Alkali Stress

Jianxin LIU, Ruirui LIU, Xiuli LIU, Haiyan JIA, Ting BU, Na LI   

  1. Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration/College of Life Sciences and Technology,Longdong University,Qingyang 745000
  • Received:2021-02-15 Online:2022-05-20 Published:2022-05-20
  • About author:LIU Jianxin(1964—),male,professor,mainly engaged in the study of physiological and ecological of plant stress.
  • Supported by:
    the National Natural Science Foundation of China(31960375);Natural Science Foundation of Gansu Province(20JR5RA491)


In order to illuminate the regulating mechanism of gas signal hydrogen sulfide(H2S) on reactive oxygen species(ROS) metabolism of naked oat(Avena nuda) under saline-alkali stress, and to screen and determine the optimal application period and concentration of H2S, the effect of spraying NaHS(H2S donor; 0, 25, 50, 100, 200 and 400 μmol·L-1) at different periods(seedling stage, jointing stage, heading stage, flowering stage and filling stage) on the H2S content, H2S production related enzyme activityof L-cysteine desulfhydrylase(LCD) and ROS metabolism-related enzyme activities and substance contents in leaves of soil-potted naked oat under 3.0 g·kg-1 salt-alkali mixed stress(NaCl∶Na2SO4∶NaHCO3∶Na2CO3 molar ratio was 12∶8∶9∶1) were carried out respectively. The results showed that spraying period, NaHS concentration and their interaction had significant effects on the content of H2S, superoxide anion(), hydrogen peroxide(H2O2), malondialdehyde(MDA), ascorbic acid(AsA) and glutathione(GSH), and the activities of LCD, superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), ascorbic acid peroxidase(APX) and glutathione reductase(GR) in naked oat leaves under salt-alkali stress respectively. Compared with spraying 0 μmol?L-1 NaHS, spraying a certain concentration of NaHS increased the contents of H2S, AsA,GSH and the activities of LCD, SOD, CAT, POD, APX and GR, and reduced the accumulation of , H2O2 and MDA respectively. However, the optimal spraying period and NaHS concentration of the above indexes were different. Comprehensive analysis of membership function showed that spraying 25-200 μmol?L-1 NaHS at the seedling stage and jointing stage had the highest the comprehensive evaluation value(D). It indicated that spraying 25-200 μmol?L-1 NaHS at the seedling-joint stage improved the ROS scavenging ability, alleviated the oxidative damage of salt-alkali stress on naked oats.

Key words: hydrogen sulfide, naked oat, saline-alkali stress, reactive oxygen species metabolism

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