Shading Effect on Morphology and Leaf Structure of Bletilla striata

doi:10.7525/j.issn.1673-5102.2021.06.016

Abstract

Abstract:

To explore the response of Bletilla striata to shading conditions， the two-year-old B. striata were cultivated under sunshade nets of 0%（as the control， CK）， 50%， 70% and 90% shading rate， the external morphology and leaf structure of B. striata were measured. The results showed that the plant height， leaf length， leaf width and leaf area were increased significantly under three shading treatments respectively， and the 50% shading treatment was the best. The stomatal density and wax particles in epidermis decreased gradually with the decrease of light intensity while the shape of chloroplast changed from nearly spherical to long fusiform. Moreover，the leaf thickness was increased， whereas the distance of adjacent vascular bundles was decreased under 50% shading treatment by the leaf cross-cutting observation. The less number of grana and thylakoid lamellae in chloroplast under CK treatment， and the more number of grana and the higher degree of thylakoid stacking in chloroplast under 50% treatment. However， there were the more stromal thylakoids and spacing between the lamellae under 70% and 90% shading treatment. These results suggested that the stomatal density，epidermis wax，mesophyll thickness， chloroplast shape，thylakoid lamellae and its thylakoid stacking might as the main structural basis factors of photosynthetic physiological response of B. striata to shading treatment.

Key words: Bletilla striata, shading, leaf micro-structure, chloroplast ultrastructure

CLC Number:

S567

Xiu-Yun YUAN, Shen-Ping XU, Yi-Ran ZHOU, Xi-Meng WNG, Bo CUI. Shading Effect on Morphology and Leaf Structure of Bletilla striata[J]. Bulletin of Botanical Research, 2021, 41(6): 974-981.

Figures/Tables 7

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处理 Treatment	叶数（个） Leaf number	株高 Height（cm）	叶长 Leaf length（cm）	叶宽 Leaf width（cm）	叶面积 Leaf area（cm²）
CK	4.67±033a	20.67±2.46b	25.03±0.15b	3.20±0.15b	62.86±2.75b
50%	4.67±0.33a	30.50±1.85a	35.13±0.48a	4.20±0.06a	115.79±0.46a
70%	4.67±0.33a	23.83±1.09ab	32.23±1.13a	4.23±0.22a	107.25±7.62a
90%	5.00±0.58a	23.67±1.09ab	33.17±1.79a	4.27±0.18a	110.75±4.44a

处理 Treatment	气孔密度 Stomic density（个/mm²）	气孔器长 Stomata length（μm）	气孔器宽 Stomata width（μm）	气孔面积 Stomic area（μm²）
CK	113.50±2.55a	40.19±0.62a	10.10±0.32a	318.62±10.81a
50%	98.99±1.99b	40.15±0.43a	9.69±0.21ab	305.54±7.88a
70%	97.03±2.30b	38.36±0.44b	9.27±0.11b	278.90±3.65b
90%	58.01±1.36c	40.17±0.56a	9.82±0.22ab	309.28±7.46a

处理 Treatment	叶片厚度 Leaf thickness （μm）	上表皮厚度 Upper epidermal thickness（μm）	下表皮厚度 Lower epidermal thickness（μm）	叶肉厚度 Mesophyll thickness（μm）	相邻维管束距离 Distance between adjacent vascular bundles（μm）
CK	229.75±3.71b	43.49±0.57a	28.09±1.12b	154.47±0.50b	502.06±23.60a
50%	265.96±7.53a	49.93±0.46a	33.42±1.22a	186.37±3.48a	393.09±33.49b
70%	233.46±1.84b	45.48±3.69a	32.27±0.59a	154.84±1.15b	446.61±23.91ab
90%	217.17±6.91b	43.03±1.59a	32.89±0.89a	146.60±5.33b	521.74±14.33a

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