Cloning and Disease Resistance Response Potential of Walnut JrNPR1 Gene

doi:10.7525/j.issn.1673-5102.2022.01.005

Abstract

Abstract:

Nonexpressor of pathogenesis-related gene 1（NPR1） played a key role in regulating plant disease resistance. Walnut（Juglans regia） was an important economic oil tree for rural revitalization in China， and its growth and yield were severely restricted by pests and diseases. In order to explore the physiological mechanism of walnut disease resistance and screen disease resistance genes， the JrNPR1 gene was cloned from ‘Xiangling’ walnut， and its basic biological information and disease response were analyzed to predict the function of JrNPR1. The results showed that the ORF of JrNPR1 gene was 1 782 bp， encoded 593 amino acids， and the theoretical isoelectric point was 6.40. Multi-sequence comparison with Morella rubra， Ricinus communis and other homologous proteins showed that they all had NPR1-like-C conserved domains， and JrNPR1 had a relatively close evolution with M. rubra and Quercus suber. The upstream 1 233 bp promoter contained a variety of cis-acting elements related to plant disease resistance， such as WRKY71OS. Under treatment with pathogens such as Colletotrichum gloeosporioides， Melanconium oblongum， and Xanthomonas campestris， JrNPR1 was significantly induced， and its expression was up-regulated by salicylic acid（SA）. The expression level of JrNPR1 under CgTJ+SA， MoZK+SA， XcHB+SA was 1.31~178.89 fold of that under single stress of CgTJ， MoZK， XcHB， respectively. These results suggested that JrNPR1 might be an important disease resistance gene against walnut anthracnose， branch blight and bacterial black spot， and might be involved in the SA signal pathway.

Key words: Juglans regia, NPR1 gene, gene expression, disease resistance

CLC Number:

S664.1

Kaiheng Ma, Jianing He, Muhong Xie, Bin Ren, Qianxue Huang, Guiyan Yang. Cloning and Disease Resistance Response Potential of Walnut JrNPR1 Gene[J]. Bulletin of Botanical Research, 2022, 42(1): 39-46.

Figures/Tables 6

Fig.1

Fig.2

Fig.3

Table 1

The cis-acting elements contained in JrNPR1 promoter

顺势作用元件 Cis-element	起始位点 Start position	序列 Sequence	特征 Characteristic
WRKY71OS	430	TGAC	W-BOX、WRKY识别，病害响应 W-BOX，WRKY binding，pathogen response
ARR1AT	122/187/192/201/339	NGATT	响应调节因子ARR1相关 Response regulator ARR1 related
ABRELATERD1	334	ACGTG	ABRE识别 ABRErecognition
ABRERATCAL	333	MACGYGB	ABRE识别 ABRErecognition
ACGTATERD1	81/334//364	ACGT	干旱早期响应 Early drought response
AMYBOX1	546	TAACARA	α-淀粉酶基因相关 Alpha-amylase gene related
CAATBOX1	57/633/761/890/932/979/1017	CAAT	组织特异表达 Tissue specific expression
OSE2ROOTNODULE	115	CTCTT
ROOTMOTIFTAPOX1	37/48/263/298/416/424/540/553/596/638/682/724/766/810/854/895/ 937/981/1022/1210	ATATT
CACTFTPPCA1	498/524/589/604/646/675/690/719/732/774/803/818/847/862/903/ 945/974/989/1060/1091/1101/1130/1145/1174/1203/1218	YACT	CACT关键组成部分 Key components of CACT
CCAATBOX1	1947	CCAAT	热激蛋白、热胁迫响应相关 Heat shock protein，heat stress response related
CURECORECR	87/523/1090/	GTAC	铜响应元件 Copper response element
DOFCOREZM	68/156/454/1087	AAAG	Dof转录因子识别 Dof transcription factor recognition
GAREAT	546	TAACAAR	诱导子响应元件 Elicitor response element
GT1CONSENSUS	214	GRWAAW	GT1结合元件、光调控 GT1 binding element，light control
GTGANTG10	5/96/329/338/429	GTGA	果胶酸裂解酶相关 Pectate Lyase related
INRNTPSADB	55/	YTCANTYY	光调控相关 Light control
-10PEHVPSBD	132/137	TATTCT
SORLIP5AT	3	GAGTGAG
NAPINMOTIFBN	269	TACACAT	2S白蛋白 2S Albumin
NODCON2GM	115	CTCTT	结瘤素Nodulin
POLASIG1	575/582/618/624/660/667/704/788/795/876/959/966/1003/1044/ 1052/1074/1115/1122/1159/1188	AATAAA	淀粉酶相关 Amylase related
POLASIG2	710/746/752/832/838/881/917/923/1009	AATTAAA
POLASIG3	309/634/762/891/933/1018	AATAAT
SEF1MOTIF	48/298	ATATTTAWW	SEF结合位点 SEF binding site
SEF4MOTIFGM7S	194/291/444	RTTTTTR	SEF4结合位点 SEF4 binding site
SURECOREATSULTR11	480	GAGAC	硫响应Sulfur response
TAAAGSTKST1	155	TAAAG	保卫细胞特异性基因表达 Guard cell specific gene expression
TATABOX2	281/571/614/656/700/742/784/828/872/913/955/999/1040/1070/ 1111/1155/1184	TATAAAT	精确起始Precise start
TATABOX4	612/698/740/826/870/911/953/997/1038/1068/1109/1153/1182	TATATAA	贮藏蛋白A基因相关 Storage protein A gene related

Table 1

Fig.4

Fig.5

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