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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (2): 316-320.doi: 10.7525/j.issn.1673-5102.2018.02.021

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The in vitro Culture for Vaccinium vitis-idaea Stem Apex

LI Yan-Xia1, DENG Ji-Feng2, LI Gui-Jun1   

  1. 1. Forestry Research Institute of Heilongjiang Province, Harbin 150081;
    2. College of Forestry, Shenyang Agricultural University, Shenyang 110866
  • Received:2017-07-31 Online:2018-03-15 Published:2018-03-23
  • Supported by:
    Heilongjiang provincial forestry bureau program(sgzjY2014008);China-Belarus-science and technology cooperation project(CB11-15)

Abstract: In order to improve the breeding rate and qualities of Vaccinium vitis-idaea seedlings, in our study, V.vitis-idaea stem apexes were used as research materials, then rapid propagation systems of plants induced by stem apex in vitro culture was established. The results indicated that the optimal induced stem apex in vitro culture is WPM(improved) +ZT 0.05 mg·L-1 +IBA 0.5 mg·L-1 +GA3 0.1 mg·L-1. The success rate of plants induced by 0.1-0.3 mm long stem apex was only 28%, while such a rate of plants induced by 0.3-0.5 mm long stem apex was up to 68%; then stem apex reached to 0.5-0.7 mm, the success rate was 79%, and the success rate of plants induced by 0.7-1.0 mm long stem apex reached up to 94%.

Key words: Vaccinium vitis-idaea L., stem apex culture, hormone, culture medium

CLC Number: