Flower Bud Differentiation and Endogenous Hormone Changes of Rosa ‘Angela’

doi:10.7525/j.issn.1673-5102.2021.01.005

Abstract

Abstract:

The experiment was conducted to explore the relationship between structure of flower buds and hormones content in Rosa ‘Angela’， and to regulate flowering， to improve landscape quality and breeding work. The flower bud differentiation was divided into five periods within 30 d. The growth cone was conical before the differentiation period. Then the cone became flat， five protrusions appeared around the growth cone at the sepal differentiation period. Multiple petal primordiums and stamen primordiums were respectively appeared around the growth cone during the flower bud differentiation. Several pistil primordiums were appeared at the base of growth cone at pistil differentiation period. The contents of ABA and CTK increased first and then decreased， which contents at sepal primordium differentiation period were significantly higher than other periods. The content of IAA increased gradually trend， and the content of GA decreased gradually. The ratio of IAA/GA and IAA/ABA showed a gradual increasing trend. The ratio of CTK/GA and（ABA+CTK）/GA at sepal primordium differentiation period were significantly higher than that before the differentiation period. The significant increasing of ABA and CTK contents at the sepal primordium differentiation period were related to flower bud induction. Low level of GA and decreasing of IAA were beneficial for flower bud differentiation. The increasing of CTK/GA and（ABA+CTK）/GA ratio at sepal primordium differentiation period were related to the flower bud induction， and high levels of IAA/ABA and IAA/GA ratio may relate to the further development of floral organ primordium.

Key words: Rose, flower bud differentiation, structure, endogenous hormone

CLC Number:

S685.12

Zhi-Yuan LIU, Li ZENG, Xi-Wu DU, PENG-Yong-Zheng, Yi-Wei TAO, Yu-Qing LI, Jun QIN. Flower Bud Differentiation and Endogenous Hormone Changes of Rosa ‘Angela’[J]. Bulletin of Botanical Research, 2021, 41(1): 37-43.

Figures/Tables 5

Table 1

Fig.1

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References 22

1	杨永花，张建旗，廖伟彪，等.叶面追肥对藤本月季“安吉拉”生长及开花的影响［J］.甘肃农业大学学报，2012，47（1）：69-72，77.
	Yang Y H，Zhang J Q，Liao W B，et al.Effects of foliage spray on growth and florescence of climbing Rosa ‘Anjila’［J］.Journal of Gansu Agricultural University，2012，47（1）：69-72，77.
2	Lang A.Physiology of flower initiation［M］.//Lang A.Differenzierung und Entwicklung/Differentiation and Development.Berlin，Heidelberg：Springer，1965：1380-1536.
3	吴莉英，唐前瑞，尹恒，等.观赏植物花芽分化研究进展［J］.生物技术通讯，2007，18（6）：1064-1067.
	Wu L Y，Tang Q R，Yin H，et al.Flower bud differentiation mechanism of ornamental plants［J］.Letters in Biotechnology，2007，18（6）：1064-1067.
4	杨艺，王奎玲，刘庆华，等.粉绿铁线莲花芽分化研究［J］.园艺学报，2019，46（8）：1565-1575.
	Yang Y，Wang K L，Liu Q H，et al.Studies on the flower bud differentiation of Clematis glauca［J］.Acta Horticulturae Sinica，2019，46（8）：1565-1575.
5	张宁，黄曜曜，敖妍，等.文冠果花芽分化过程及内源激素动态变化［J］.南京林业大学学报：自然科学版，2019，43（4）：33-42.
	Zhang N，Huang Y Y，Ao Y，et al.Flower bud differentiation and dynamic changes of endogenous hormone in Xanthoceras sorbifolium Bunge［J］.Journal of Nanjing Forestry University：Natural Sciences Edition，2019，43（4）：33-42.
6	马焕普.果树花芽分化与激素的关系［J］.植物生理学通讯，1987（1）：1-6.
	Ma H P.The relationship between flower initiation of fruit trees and hormones［J］.Plant Physiology Communications，1987（1）：1-6.
7	贺海洋，朱金启，高琪洁，等.单叶蔷薇的花芽形态分化［J］.园艺学报，2005，32（2）：331-334.
	He H Y，Zhu J Q，Gao Q J，et al.Morphological differentiation of the flower bud of Rosa persica［J］.Acta Horticulturae Sinica，2005，32（2）：331-334.
8	马子骏，常毅，侯世昌.苦水玫瑰的花芽分化研究［J］.园艺学报，1986，13（2）：131-134，145.
	Ma Z J，Chang Y，Hou S C.Studies on the differentiation of the flower bud of Kushui rose［J］.Acta Horticulturae Sinica，1986，13（2）：131-134，145.
9	范天刚，张钢，田亚然，等.低温诱导切花月季过度重瓣化的形态学观察［J］.东北林业大学学报，2014，42（9）：116-121.
	Fan T G，Zhang G，Tian Y R，et al.Morphological observation of excessive petal Rosa hybrida induced by low temperature［J］.Journal of Northeast Forestry University，2014，42（9）：116-121.
10	胡祖坤.月季花芽的形态建成及生殖过程［J］.园艺学报，1988，15（4）：269-271，292-295.
	Hu Z K.The form-formation of flower bud and sexual reproductive process in Rosa polyantha Hort［J］.Acta Horticulturae Sinica，1988，15（4）：269-271，292-295.
11	Lyndon R F.Flower development in Silene：morphology and sequence of initiation of primordia［J］.Annals of Botany，1978，42（6）：1343-1348.
12	王瑞勤，李忠喜.玫瑰花芽分化的研究［J］.北京林业大学学报，1992，14（1）：39-45.
	Wang R Q，Li Z X.Studies on flower bud differentiation of Rosa rugosa cv.‘Plena’［J］.Journal of Beijing Forestry University，1992，14（1）：39-45.
13	Maas F M，Hofman-Eijer L B，Hulsteijn K.Flower morphogenesis in Rosa hybrida ‘Mercedes’ as studied by cryo-scanning electron and light microscopy.Effects on light and shoot position on a branch［J］.Annals of Botany，1995，75（2）：199-205.
14	曾骧.果树生理学［M］.北京：北京农业大学出版社，1992.
	Zeng X.Fruit tree physiology［Z］.Beijing：Beijing Agricultural University Publishing House，1992.
15	曹尚银，张俊昌，魏立华.苹果花芽孕育过程中内源激素的变化［J］.果树学报，2000，17（4）：244-248.
	Cao S Y，Zhang J C，Wei L H.Studies on the changes of endogenous hormones in the differentiation period of flower bud in apple trees［J］.Journal of Fruit Science，2000，17（4）：244-248.
16	李秉真，孙庆林，张建华，等.苹果梨花芽分化期内源激素含量的变化（简报）［J］.植物生理学通讯，2000，36（1）：27-29.
	Li B Z，Sun Q L，Zhang J H，et al.The changes of endogenous hormones in apple-pear during flower bud formation［J］.Plant Physiology Communications，2000，36（1）：27-29.
17	程洪，黄辉白.柑桔成花机理的研究：Ⅱ.与核酸代谢的关系［J］.果树科学，1992，9（2）：70-76.
	Cheng H，Huang H B.Studies of the mechanism of citrus flower-bud formation Ⅱ.relations to nucleic acid metabolism［J］.Journal of Fruit Science，1992，9（2）：70-76.
18	樊卫国，刘国琴，安华明，等.刺梨花芽分化期芽中内源激素和碳、氮营养的含量动态［J］.果树学报，2003，20（1）：40-43.
	Fan W G，Liu G Q，An H M，et al.Study on the changes of endogenous hormones，carbohydrate and nitrogen nutrition at the flower bud differentiation stage of Rosa roxburghii［J］.Journal of Fruit Science，2003，20（1）：40-43.
19	Costantini E，Landi L，Silvestroni O，et al.Auxin synthesis-encoding transgene enhances grape fecundity［J］.Plant Physiology，2007，143（4）：1689-1694.
20	Noodén L D，Thimann K V.Evidence for a requirement for protein synthesis for auxin-induced cell enlargement［J］.Proceedings of the National Academy of Sciences of the United States of America，1963，50（2）：194-200.
21	李建安，孙颖，郜爱玲，等.油桐花芽分化期营养与激素生理变化研究［J］.中南林业科技大学学报，2011，31（3）：34-37，53.
	Li J A，Sun Y，Gao A L，et al.Physiological nutrition and hormone variations in Vernicia fordii during flower bud differentiation［J］.Journal of Central South University of Forestry & Technology，2011，31（3）：34-37，53.
22	梅虎，谈锋.紫苏花芽生理分化期叶片内源激素含量变化的动态研究［J］.西南师范大学学报：自然科学版，2002，27（2）：206-209.
	Hu M，TAN F.Endogenous hormones metabolism in leaves during the physhiological flower bud differentiation phase of Perilla frutescens（L.） Britte［J］.Journal of Southwest China Normal University：Natural Science，2002，27（2）：206-209.

采样观察时间 Observation time (y.m.d)	花芽分化过程中各时期花芽数 Number of flower buds in each period of flower bud differentiation
采样观察时间 Observation time (y.m.d)	Ⅰ	Ⅱ	Ⅲ	Ⅳ	Ⅴ
2019.03.20	20
2019.03.25	12	6	2
2019.04.04		3	7	7	3
2019.04.09			3	5	12
2019.04.14				2	18
2019.04.19					20

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