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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (3): 380-387.doi: 10.7525/j.issn.1673-5102.2016.03.010

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Cloning and Expression Analysis of a Plasma Membrane Na+/H+ Antiporter Gene in Tamarix hispida

JIA Yuan-Yuan, ZHANG Chun-Rui, WANG Yu-Cheng, YANG Chuan-Ping, WANG Chao   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2015-11-13 Online:2016-05-15 Published:2016-05-20

Abstract: A full length cDNA of a Na+/H+ antiporter gene (named ThSOS1) gene was isolated from the transcriptome cDNA librarys of Tamarix hispida. ThSOS1 was 3 917 bp in length, including an open reading frame of 3 498 bp which was predicted to encode a polypeptide of 1 165 amino acids. The estimated molecular weight and isoelectric point of the putative protein were 128.8 kDa and 6.42, respectively. By hydrophobic cluster analysis, ThSOS1 contained 10 potential transmembrane domains within the N- terminal protion and a long hydrophilic cytoplasmic tail in the C-terminal protion. By multiple sequence alignment, ThSOS1 show 92%, 73%, and 72% identities in amino acid sequence to plasma membrane Na+/H+ antiporter genes from Reaumuria trigyna, Chenopodium quinoa and Suaeda salsa. By phylogenetic analysis, ThSOS1 was more related to the plasma membrane-type Na+/H+ antiporter and clustered distantly with the vacuolar-typed Na+/H+ antiporter. By quantitative real-time PCR assay, the mRNA levels of ThSOS1 was significantly up-regulated in T.hispida under NaCl and PEG treatments. Therefore, ThSOS1 might play an important role in salt and drought tolerance of T.hispida.

Key words: Tamarix hispida, Na+/H+ antiporter, stress responses, gene expression

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