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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (1): 113-122.doi: 10.7525/j.issn.1673-5102.2019.01.014

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Cloning and Expression Analysis of S-Adenosyl-L-Methionine Synthetase(ThSAMS) Gene from Tamarix hispida

ZHANG Yue1, ZHAO Xin1, HOU Zheng1, WANG Yan-Min1,2, WANG Yu-Cheng1, WANG Chao1   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
    2. Forestry Science Research Institute of Heilongjiang Province, Harbin 150081
  • Received:2018-08-28 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    National Natural Science Foundation of Heilongjiang Province(QC2016019);National Natural Scientists Fund(31300571)

Abstract: A full length cDNA of a S-Adenosyl-L-Methionine Synthetase gene(named ThSAMS) was isolated from the transcriptome cDNA librarys of Tamarix hispida. The open reading frame of ThSAMS cDNA was 1 185 bp encoding 394 amino acids. The relative molecular weight of ThSAMS protein was 97.85 kDa, with isoelectric point of 5.02. Multiple alignment analysis based on the amino acids indicated that ThSAMS had high homology to SAMS proteins from different species, and the highest similarity of 95% to the Ziziphus jujuba SAMS. Quantitative real-time PCR(qRT-PCR) assay revealed that ThSAMS gene can respond to NaCl, PEG and ABA stress, suggesting that it may be involved in salt and drought stresses in T.hispida. This study provided theoretical groundwork for further exploration into the function of stress tolerance of SAMS gene in plants.

Key words: Tamarix hispida, ThSAMS gene, S-Adenosyl-L-Methionine Synthetase, gene expression

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