Cloning and Sequence Analysis of bHLH Gene from Taxus chinensis var. mairei

doi:10.7525/j.issn.1673-5102.2015.01.010

Abstract

Abstract: We cloned a new bHLH transcription factor named TcMYC by RT-PCR technology from Taxus chinensis var. mairei, with GenBank accession number KC878013. By the Blast analysis, it belongs to MYC subgroup. By the bioinformatic analysis, the sequences length of TcMYC is 1 959 bp encoding one opening reading frame with 650 amino acid residues with hydrophilic property. The molecular weight of TcMYC is 71.4 kD, and the theoretical pI is 4.87. The predicated protein TcMYC is localized to cell nucleus, as well as contained one helix-loop-helix structure. By Blast and multiple sequences alignment analysis, TcMYC shares 98%, 45% and 44% identity with Taxus cuspidata, Vitis vinifera and Nicotiana tabacum, respectively. The constructed tree shows that the MYC transcription factors of the plants belonging to the same family clustered together, and as Taxus is a gymnosperms plant, TcMYC and TaMYC are not clustered with other angiosperms plants. The expression of TcMYC is a little declined in Methyl jasmonate induced Taxus cell culture. TcMYC may regulate Taxol biosynthesis in negative.

Key words: Taxus, bHLH, gene cloning, bioinformatics, taxol

CLC Number:

ZHOU Hua1；ZHU Qi1；YANG Yan-Fang2*；LIU Hong-Wei2；YU Fa-Xin1；QIU De-You2. Cloning and Sequence Analysis of bHLH Gene from Taxus chinensis var. mairei[J]. Bulletin of Botanical Research, 2015, 35(1): 52-59.

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