Constructing and Analyzing SSH Library for the Early Response Genes in Wheat Brock after Blumeria graminis f. sp. tritici Stress

doi:10.7525/j.issn.1673-5102.2015.01.011

Abstract

Abstract: We constructed a suppression subtractive hybridization (SSH) library for the early response genes in wheat Brock under Blumeria graminis f. sp. tritici(Bgt) stress by using the cDNA from Bgt-inoculated Brock leaf as the tester and the cDNA from Bgt-uninoculated Brock leaf as the driver. Fifty positive clones were picked randomly and sequenced, 38 redundant sequences were acquired. By sequence comparison against the GenBank database, 28 sequences was homolog of known genes. The transcriptional levels were assayed by fluorescence quantitative PCR, and the ABC transporter, serine/threonine protein kinase, Lr1 gene-related protein, Ribulose bisphosphate carboxylase and Chlorophyll a/b binding protein were involved in the early response to Bgt stress in wheat cultivar Brock. Our work was useful for the powdery mildew resistance mechanism in wheat.

Key words: cultivar wheat Brock, powdery mildew, suppression subtractive hybridization(SSH), expression sequence tag(EST)

CLC Number:

QIAO Shi-Yao；LIU Xiao-Ying；WANG Yan-Hong；XIAO Ying；SUN Shi-Nan；WANG Zhen-Ying*. Constructing and Analyzing SSH Library for the Early Response Genes in Wheat Brock after Blumeria graminis f. sp. tritici Stress[J]. Bulletin of Botanical Research, 2015, 35(1): 60-67.

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