Fig.1

Effect of different sample preparation methods on the morphology of stigma papilla cells of cultivated jujube ‘Junzao’A.Natural air-drying method； B.Freeze-drying method； C.Carbon dioxide critical point drying method.

Fig.2

Effect of different acceleration voltages（AV） on the morphology of stigma papilla cells of cultivated jujube ‘Junzao’A.AV=1 kV； B.AV=3.0 kV； C.AV=5.0 kV.

Fig.3

Effect of different working distances on the morphology of stigma papilla cells of cultivated jujube ‘Junzao’A.WD=21.2 mm；B.WD=10.8 mm；C.WD=5.4 mm.

Fig.4

The stigma morphology of cultivated jujube ‘Junzao’ observed by SEMA.Floral organs of cultivated jujube ‘Junzao’；B.The micrograph of the enlarged bifurcated type stigma within the red square area of Fig.4A；C.Morphology of the papilla cells；D.Proteins on the surface of papilla cells；E.Contents of the papilla cells；F.Enlarged view of the contents of the papilla cells. The red square indicated jujube stigma. Red arrow represented jujube stigma papilla cells； black arrow represented jujube style cells.

Fig.5

Pollen-stigma interaction between self-pollination and cross-pollination in jujube observed by SEMA.Pollen grain morphology of cultivated jujube ‘Junzao’；B.Pollen grain morphology of sour jujube ‘Qingjian Suanzao’；C.At 0 h after self-pollination，pollen grains were being in adhered and hydrated status；D.At 0 h after cross-pollination，pollen grains were being in adhered and hydrated status；E.At 3 h after self-pollination，pollen grains were germinated，and papilla cells were wilted；F.At 3 h after cross-pollination，pollen grains were germinated，and papillae cells were wilted；G.At 12 h after self-pollination，pollen tubes grew，and papillae cells were damaged；H.At 12 h after cross-pollination，pollen tubes grew，and papillae cells were damaged；I.At 24 h after self-pollination，pollen tubes grew through the papillae cells into the style；J.At 24 h after cross-pollination，pollen tubes grew through the papillae cells into the style.

Fig.6

Fluorescence microscopic observation of pollen tube growth in self-pollination and cross-pollination of jujubeA.At 12 h after self-pollination，the pollen tube elongated to 1/3 of the style；B.At 12 h after cross-pollination，the pollen tube elongated to 1/3 of the style；C.At 24 h after self-pollination，the pollen tube elongated to the middle of the style and stopped growing；D.At 24 h after cross-pollination，the pollen tube elongated to the base of the style.