Germplasm Innovation and Characteristic Analysis of Transgenic PsnNAC007Populus simonii×P. nigra with High Drought Tolerance

doi:10.7525/j.issn.1673-5102.2024.03.004

Abstract

Abstract:

Populus simonii×P. nigra is a fast-growing and cold-tolerant tree species with elite wood properties in northeast China. In order to produce a new forest germplasm with more widely planting and improved drought tolerance， this study successfully generated transgenic P. simonii×P. nigra using PsnNAC007， a key drought response transcription factor gene. Growth characteristics， drought tolerant ability， net photosynthetic rate（P_n）， transpiration rate（T_r）， stomatal conductance（G_s）， water conductance， cell morphology and wood components of overexpressed PsnNAC007 transgenic plants were analyzed respectively. The results showed that there was no significant difference in growth between transgenic and wild-type plants， but drought survival rate was increased by 26.15%. Under drought condition， transgenic plants exhibited reduced Cleaf and T_r， improved water use efficiency， and lower water conductance loss. Anatomical analysis showed that the overexpression of PsnNAC007 led to more and smaller stem xylem vessels， which were conducive to the continuous and efficient water transport in plants under drought conditions. Based on wood components analysis， lignin deposition in stems of transgenic plants was increased significantly， and monosaccharides that constituted cellulose and hemicellulose showed no significant change.

Key words: Populus simonii × P. nigra, drought stress, transcription factor, genetic transformation

CLC Number:

S792.11

Xiaoqian WU, Xu HE, Jinghui GAO, Shuang LI. Germplasm Innovation and Characteristic Analysis of Transgenic PsnNAC007Populus simonii×P. nigra with High Drought Tolerance[J]. Bulletin of Botanical Research, 2024, 44(3): 349-360.

Figures/Tables 12

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引物名称 Primer name	引物序列（5′→3′） Sequences（5′→3′）	用途 Application
PsnNAC007-F	CACCATGAAAGGAAATAGATCAGCAG	PsnNAC007基因克隆 PsnNAC007 gene cloning
PsnNAC007-R	GCACACATCAAGAACCCTATT	PsnNAC007基因克隆 PsnNAC007 gene cloning
PsnNAC007-XbaⅠ-F	CTAGTCTAGAATGAAAGGAAATAGATCAGCAG	pBI121-35S-PsnNAC007载体构建 pBI121-35S-PsnNAC007 vector construction
PsnNAC007-SacⅠ-R	CTAGGAGCTCGCACACATCAAGAACCCTATT
35S-F	CCCACTATCCTTCGCAAGACC	转基因鉴定 Transgenic identification
PsnNAC007-middle-R	CGACCAATGGGTTTATCTGCTC	转基因鉴定 Transgenic identification
RT-PsnActin-F	GGATATTCAGCCCCTTGTCTG-	相对表达量检测引物 Relative expression level detection primers
RT-PsnActin-R	TCGTCACCAACATAAGCATCC
RT-PsnNAC007-F	ATGAAAGGAAATAGATCAGCAGAT
RT-PsnNAC007-R	ATTGGCCTCCACATTTCTTAAGC

参数 Parameter	野生型 Wild-type/%	PsnNAC007过表达植株 OE-PsnNAC007/%
不可溶性木质素 Acid-insoluble lignin	20.32±0.23	22.43±0.28**
可溶性木质素 Acid-soluble lignin	2.96±0.07	2.64±0.03**
总木质素Total lignin	23.29±0.29	25.07±0.30**
葡萄糖Glucose	53.37±0.17	53.63±0.19
木糖Xylose	11.39±0.26	11.59±0.40
半乳糖Galactose	1.34±0.05	1.28±0.03
阿拉伯糖Arabinose	2.30±0.13	2.29±0.05
总碳水化合物 Total carbohydrate	68.41±0.38	68.79±0.48

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