Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (3): 492-501.doi: 10.7525/j.issn.1673-5102.2022.03.019

• Physiology and Ecology • Previous Articles     Next Articles

The Effect of Exogenous Proline on the Stomata of Nitraria tangutorum Leaves under Natural Drought

Bin WEI, Yi LI, Shiping SU()   

  1. College of Forestry,Gansu Agricultural University,Lanzhou 730070
  • Received:2021-03-21 Online:2022-05-20 Published:2022-05-20
  • Contact: Shiping SU E-mail:susp008@163.com
  • About author:WEI Bin(1993—),male,master student,majoring in the research of germplasm resources.
  • Supported by:
    Forestry First-level Discipline Construction fund of Gansu Agricultural University(GAU-XKJS-2018-103);National Natural Science Foundation of Cina(32060335)


Taking Nitraria tangutorum, a perennial desert plant in the field environment, as material, scanning electron microscope was used to observe the microstructure of N. tangutorum leaf stomata under different exogenous proline concentrations, and the length, width, area and density of the leaf epidermal stomatal apparatus were measured respectively, and the difference of the same index under different proline concentration treatments were compared. The concentrations of exogenous proline spraying were set at 50, 100, 150, 200, 250 mg·L-1 respectively(Proline, abbreviated as Pro or P) under natural drought stress, and the drought tolerance of N. tangutorum were tested respectively. The observation results showed that the stomata guard cells of N. tangutorum leaves were kidney-shaped, and the stomata were randomly distributed in the leaf epidermis. The stomatal apparatus was mostly irregular, and the stomata were oval and leaf surface waxy less. And the leaf cells appeared folds and sinks. Compared with the control, under different concentration treatments and different sampling days, the stomata length, width and area decreased, and the stomata density increased. As the concentration increased and the number of sampling days changed, the overall stomata length showed a downward trend. When the concentration reached at 200 mg·L-1, the decreasing amplitude increased, and the overall width and area showed a trend of first decreased, then increased and then decreased, while the stomata density showed an overall upward trend. Among them, the stomata length, width and area were at a concentration of 50 mg·L-1, the number of sampling days reached the maximum on the first day, and the stomatal density reached the maximum at the concentration of 150 mg·L-1 and the ninth day of the sampling days. In addition, the stomata length differed significantly on the first day, the sixth day, and the ninth day under the treatment of different concentrations of proline, while the stomata width and area were not significantly different. Except for the beginning, the stomata density was different due to the different proline concentration. The difference was significant(P<0.05). The results showed that spraying different concentrations of exogenous proline on N. tangutorum leaves under natural drought stress could reduce the length, width and area of the stomata, and increase the density of stomata, could provide a theoretical basis for N. tangutorum drought resistance.

Key words: drought stress, Nitraria tangutorum, exogenous proline, leaf surface, stomata microstructure

CLC Number: