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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 211-223.doi: 10.7525/j.issn.1673-5102.2022.02.006

• Molecular biology • Previous Articles     Next Articles

Bioinformatic Analysis and Expression Pattern of LTP Family Genes in Populus davidiana × P. alba var. pyramidalis

Qian Sun2, Yuhang Wu2, Yaxuan Zhang2, Jingdan Cao2, Jingjing Shi1, Chao Wang1()   

  1. 1.State Key Laboratory of Forest Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
    2.School of Forestry,Northeast Forestry University,Harbin 150040
  • Received:2021-07-21 Online:2022-03-20 Published:2022-02-22
  • Contact: Chao Wang E-mail:wangchao@nefu.edu.cn
  • About author:Sun Qian(2000—),female,undergraduate,major in forest genetics and breeding.
  • Supported by:
    The undergraduate innovation project of Northeast Forestry University(202010225005)


To lay a foundation for studying the molecular regulation mechanism of LTP family gene and molecular genetic improvement of Populus davidiana × P. alba var. pyramidalis, the expression pattern of LTP family genes from P. davidiana × P. alba var. pyramidalis was analyzed.The protein properties, multiple sequence alignments and phylogenetic tree analysis of LTP family members were performed to show their structural features. The P. davidiana × P. alba var. pyramidalis seedlings treated with gravity, salt and PEG stress respectively were subjected to quantitive real time PCR analysis. The expression patterns of PdbLTP family genes were carried out respectively. Eight PdbLTP genes and two subfamily PdbGLTP genes were obtained, and their CDS lengths were ranged from 294-396 bp. LTP family were hydrophobic proteins and had eight cysteine conserved structures, without transmembrane region. qRT-PCR analysis showed that the expression patterns of different PdbLTP family genes were different under gravity processing. Expression levels of PdbLTP1PdbLTP3PdbLTP5 and PdbLTP7 genes were up-regulated in tension wood compared to those in the normal wood. The transcripts levels of PdbLTP1PdbLTP2PdbLTP3PdbLTP5 genes were abundant in stem.Expression of PdbLTP family genes was induced by salt stress except for PdbLTP5. PdbLTP1PdbLTP2PdbLTP3 and PdbLTP5 were up-regulated under drought stress. Different members of PdbLTP gene family play important roles in regulation of xylem development and tolerance to abiotic stress in Populus davidiana × P. alba var. pyramidalis.

Key words: Populus davidiana×P. alba var. pyramidalis, LTP family, bioinformatics, expression pattern analysis

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