Expression and Functional Analysis of FT Homologous Genes in Saffron(Crocus sativus L.)

doi:10.7525/j.issn.1673-5102.2022.02.007

Abstract

Abstract:

As one of the three floral pathway integrators， FT（FLOWERING LOCUS T） and its homologous genes were considered to be important genes which related to flowering. In order to clarify the function of FT homologous genes and flowering mechanism of saffron（Crocus sativus L.）， the three reported FT homologous genes， named CsatFT1， CsatFT2 and CsatFT3， were isolated and analyzed. The gDNA contained the ORF with length of 835， 1 642 and 1 132 bp， respectively， and they all had 4 exons and 3 introns. cDNA sequences contained the length of 528， 525 and 540 bp ORF， which encoded 175， 174 and 179 amino acids， respectively. The phylogenetic analysis indicated that CsatFT1， CsatFT2 and CsatFT3 showed relatively close genetic distance with the monocotyledon Narcissus tazetta NtFT， Lilium longiflorum LlFT and Allium cepa AcFT1， respectively. qRT-PCR results indicated that the expression levels of CsatFT1， CsatFT2 and CsatFT3 were the highest in the leaves， followed by the lateral roots， and almost no expressed in daughter corms and primary roots at the early stage of replacement corm enlargement. At the late stage of replacement corm enlargement， the expression levels of CsatFT1， CsatFT2 and CsatFT3 were all high in daughter corms and were almost not detected in terminal buds. During indoor storage stage， the expression levels of CsatFT1， CsatFT2 and CsatFT3 were high in the capital， followed by the leaves， and almost undetectable in the petals and anthers. From the phenotypes of transgenic tobacco and transgenic Arabidopsis plants， the results showed that CsatFT1， CsatFT2 and CsatFT3 could promote the early flowering of plants.

Key words: Saffron（Crocus sativus L.）, floral induction, FT homologous gene, gene function

CLC Number:

Q785

Zhen Wang, Liuyan Yang, Weizhong Pei, Xin Li, Zhen Yang, Yongchun Zhang. Expression and Functional Analysis of FT Homologous Genes in Saffron(Crocus sativus L.)[J]. Bulletin of Botanical Research, 2022, 42(2): 224-233.

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名称 Name	正向引物 Forward primer（5′—3′）	反向引物 Reverse primer（5′—3′）
CsatGAPDH	ATAGCTTATTATTAGACGGA	CTCAAGGGAATCATGGGCT
CsatFT1	AATGAGTAGGGATTCGCTGGTG	GCGGGTTACTTGGATTTGGA
CsatFT2	AGAGTCCACGACCGTCGCTC	TAAATCCTTCTCCCACCGCA
CsatFT3	GTTTATTACAACTGCCAGAGGGAG	AAGGCTTTGGAAGAATGATTTCAAG

名称 Name	正向引物 Forward primer（5′—3′）	反向引物 Reverse primer（5′—3′）	长度 Length /bp
NtEF1α	GAGGCACTTCCTGGTGACAAT	GGGCTCCTTCTCAATCTCCTTAC	273
AtEF1α	CAAGATGGATGCCACTACCC	AGTGGGAGACGAAGGGGCT	255
CsatFT1	ATTCGCTGGTGCTCGGTAG	CCTCAGATGCGGGTTACTTG	230
CsatFT2	GGTCGGAAATGGATGTGAG	CTCTCGTAGCACACAATCTCCTG	222
CsatFT3	GAGATTGGTGGCTGTGACTAT	TGCTGGAACAGGATAAACACG	218

	数量 No.	开花时主杆叶片数量 No. leaves on main stem at flowering	现蕾时植株高度 Plant height at first flower bud /cm	从移栽到现蕾的时间 Time between transformed plantlet regeneration and first flower bud /d
Wt	6	13.70±1.34a	29.10±1.86b	68.10±1.86a
35S：：CsatFT1	25	13.20±1.40a	32.00±3.20a	59.90±3.21c
35S：：CsatFT2	20	13.10±1.20a	32.30±2.91a	61.80±3.79b
35S：：CsatFT3	25	12.50±1.35a	28.90±2.85b	59.50±2.37c

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