Effects of Carbon Source and Other Factors on the Proliferation of Embryogenic Callus of Elite Clone of Pinus elliottii

doi:10.7525/j.issn.1673-5102.2022.01.003

Abstract

Abstract:

To explore the effects of carbon sources and other main factors on the proliferation of embryogenic callus in Pinus elliottii， and provide technical methods and theoretical basis for further improving proliferation efficiency. The embryogenic callus of the elite clone PEE3-13 was used， the effects of different types and concentrations of carbon sources， pH and the organic nitrogen sources， L-glutamine and its substitutes， L-alanine-L-glutamine on its proliferation were studied. The pH and the type of carbon source had significant effects on its proliferation. The autoclaved medium pH ranged from 5.68 to 6.28， it was the most suitable for the embryogenic callus growth. The maximum proliferation rate was achieved when using 30 g·L^-1 white sugar was used as carbon source. When 450 mg·L^-1 L-glutamine was added， the embryogenic callus had the highest proliferation rate and the best activity， however， L-alanine-L-glutamine could not replace L-glutamine. The optimal medium was 30 g·L^-1 white sugar and 450 mg·L^-1 L-glutamine and autoclaved pH was ranged from 5.68 to 6.28 and was most suitable for embryogenic callus proliferation， and the maximum proliferation rate was h 851.27%.

Key words: Pinus elliottii, embryogenic callus, proliferation culture, carbon source, pH, L-glutamine

CLC Number:

S722

Qiyang Gao, Yulong Huang, Wenbing Guo, Fencheng Zhao, Yang Liu. Effects of Carbon Source and Other Factors on the Proliferation of Embryogenic Callus of Elite Clone of Pinus elliottii[J]. Bulletin of Botanical Research, 2022, 42(1): 21-28.

Figures/Tables 5

Fig.1

Table 1

Table 2

Fig.2

References 23

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灭菌前pH pH before autoclaving	灭菌后pH pH after autoclaving	平均培养前质量 Average weight before cultivation /g	平均培养后质量 Average weight after cultivation /g	平均增殖率 Average proliferation rate /%
4.0	4.77	0.75±0.04	2.48±0.01d	231.76±11.81d
5.0	5.39	0.74±0.02	3.37±0.26bc	355.36±40.52bc
6.0	5.68	0.74±0.02	3.97±0.35a	436.20±30.75a
7.0	6.28	0.75±0.04	3.73±0.48ab	398.73±47.44ab
8.0	6.82	0.75±0.02	3.08±0.24cd	309.16±33.00cd

碳源 Carbon source	平均培养前质量 Average weight before cultivation /g	平均培养后质量 Average weight after cultivation /g	平均增殖率 Average proliferation rate /%
30 g·L^-1蔗糖 30 g·L^-1 sucrose	0.75±0.01	5.13±0.48b	587.82±63.07b
20 g·L^-1蔗糖 20 g·L^-1 sucrose	0.77±0.03	3.62±0.34b	372.71±42.05b
30 g·L^-1红块糖 30 g·L^-1 brown sugar	0.76±0.03	1.47±0.38c	92.82±46.27c
45 g·L^-1麦芽糖 45 g·L^-1 maltose	0.76±0.03	0.99±0.15c	29.51±19.47c
20 g·L^-1蔗糖+10 g·L^-1麦芽糖 20 g·L^-1 sucrose+10 g·L^-1 maltose	0.75±0.04	5.32±0.73b	607.73±93.27b
30 g·L^-1白砂糖 30 g·L^-1 white sugar	0.75±0.02	7.13±0.25a	851.27±22.10a

处理 Treatment	平均培养前质量 Average weight before cultivation /g	平均培养后质量 Average weight after cultivation /g	平均增殖率 Average proliferation rate /%
未添加谷氨酰胺或丙谷二肽 Without L-alanine-L-glutamine or L-glutamine	0.75±0.01	2.05±0.11ab	174.95±14.61ab
450 mg谷氨酰胺 450 mg L-glutamine	0.76±0.01	2.12±0.12a	181.80±15.89a
670 mg丙谷二肽 670 mg L-alanine-L-glutamine	0.76±0.01	1.63±0.14b	115.19±17.70c
870 mg丙谷二肽 870 mg L-alanine-L-glutamine	0.76±0.01	1.64±0.09b	118.12±12.10bc

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