Comparison between Betalain and Flavonol Accumulation in Tricolor Bougainvillea peruviana ‘Thimma’ Based on Transcriptome

doi:10.7525/j.issn.1673-5102.2017.02.013

Abstract

Abstract: Bougainvillea peruviana ‘Thimma’, accumulating betalains, not anthocyanins occurring in majority of plants. The cultivar was characterized with either white, magenta, and white-magenta(variegated) bracts in the same branches. We first used magenta(Yp), bicolor(Ym) and white(Yw) open inflorescences for transcriptome analysis by sequencing. Candidate genes involving in pigment metablisms were validated by real-time PCR. We obtained 73,325 genes with average length of 616 bp. Out of DEGs, 327 candidate genes of betalain-biosynthesis, 308 flavonoid-biosynthesis genes and 466 anthocyanin-accumulation genes were detected. Eight candidate pigment-related genes were verified, four were responsible for betalain production(PPO, CYP76AD1, cDOPA-5-GT, DODA), and four responsible for flavonoids production(FLS, DFR, LDOX, 3-GT). Expression strength of four betalain production genes was well consistent with betalain-accumulation in Yp, Ym and Yw. Full later three anthocyanidin-producing genes(DFR, LDOX, 3-GT) were first verified and the expression varied in three samples. Expression of all eight candidate genes was well consistent with RNA-sequencing data. B.peruviana could provide a good tool to study physiological, phytochemical and molecular mechanisms in Bougainvillea, effectively eliminating the interference of some factors.

Key words: Bougainvillea peruviana, transcriptome, real-time PCR, betalain, flavonol

CLC Number:

XU Su-Xia, HUANG Qing-Yun, LIN Chun-Song, HUANG Yi-Jin, HU Ou. Comparison between Betalain and Flavonol Accumulation in Tricolor Bougainvillea peruviana ‘Thimma’ Based on Transcriptome[J]. Bulletin of Botanical Research, 2017, 37(2): 249-258.

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