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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (2): 249-258.doi: 10.7525/j.issn.1673-5102.2017.02.013

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Comparison between Betalain and Flavonol Accumulation in Tricolor Bougainvillea peruviana ‘Thimma’ Based on Transcriptome

XU Su-Xia1, HUANG Qing-Yun1, LIN Chun-Song1, HUANG Yi-Jin2, HU Ou1   

  1. 1. Key Laboratory of Plant Physiology & Biochemistry, Fujian Institute of Subtropical Botany, Xiamen 361006;
    2. The First Affiliated Hospital of Xiamen University, Xiamen 361003
  • Received:2016-07-11 Online:2017-03-15 Published:2017-03-11
  • Supported by:
    The National Natural Science Foundation of China(Grant No.31071814;31372093);Xiamen Creation Plantform of Science&Technology(3502Z20131004)

Abstract: Bougainvillea peruviana ‘Thimma’, accumulating betalains, not anthocyanins occurring in majority of plants. The cultivar was characterized with either white, magenta, and white-magenta(variegated) bracts in the same branches. We first used magenta(Yp), bicolor(Ym) and white(Yw) open inflorescences for transcriptome analysis by sequencing. Candidate genes involving in pigment metablisms were validated by real-time PCR. We obtained 73,325 genes with average length of 616 bp. Out of DEGs, 327 candidate genes of betalain-biosynthesis, 308 flavonoid-biosynthesis genes and 466 anthocyanin-accumulation genes were detected. Eight candidate pigment-related genes were verified, four were responsible for betalain production(PPO, CYP76AD1, cDOPA-5-GT, DODA), and four responsible for flavonoids production(FLS, DFR, LDOX, 3-GT). Expression strength of four betalain production genes was well consistent with betalain-accumulation in Yp, Ym and Yw. Full later three anthocyanidin-producing genes(DFR, LDOX, 3-GT) were first verified and the expression varied in three samples. Expression of all eight candidate genes was well consistent with RNA-sequencing data. B.peruviana could provide a good tool to study physiological, phytochemical and molecular mechanisms in Bougainvillea, effectively eliminating the interference of some factors.

Key words: Bougainvillea peruviana, transcriptome, real-time PCR, betalain, flavonol

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