Abstract: Plant Metallothionein type2 isolated from Brassica juncea was transformed into tobacco leaves via agrobacterium tumefaciens-mediated transformation. The transformation was confirmed by PCR and Western blot analysis. The transgenic plants differentiated in their growing phenotypes and in root elongation compared with those of wild types. The level of GSH in transformed and wild type of tobacco leaves did not show significant variation but increased extremely in the transformed leaves after 100 mmol·L^-1 Cu²⁺ stress. The analysis of ATP hydrolysis showed that the P-ATPase activities were higher in the microsomal fraction prepared from transformed tobacco leaves than those from wild types. However, the V-ATPase activities were declined in comparison with the wild types.

Key words: Brassica juncea, GSH, Metallothionein type2, tobacco, P-ATPase, V-ATPase