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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (4): 547-553.doi: 10.7525/j.issn.1673-5102.2015.04.012

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Function of Non-specific Lipid Transfer Protein Gene PsnsLTP in Polygonum sibiricum Laxm

LI Wei1;QU Chun-Pu1;XU Zhi-Ru1,2;LIU Chun1;LIU Guan-Jun1*   

  1. 1.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040;
    2.College of Life Science,Northeast Forestry University,Harbin 150040
  • Online:2015-07-20 Published:2016-03-09
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Abstract: We transformed the plant expression vector pROKII-PsnsLTP harboring non-specific lipid transfer protein gene from Polygonum sibiricum Laxm into tobacco by Agrobacterium-mediated genetic method. After transgenic tobacco was identified by kanamycin resistance screening and PCR, we observed phenotypic after induced Colletotrichum nicotianae and Pythium aphanidermatum, respectively, on the leaves of transgenic tobacco and wild type, and measured physiological indicators, the phenotypic in transgenic tobacco, and wild type in the treatments of NaCl, CdCl2, 7.5% PEG6000 solutions. Compared with wild type tobacco, transgenic tobacco plants had a strong resistance to Colletotrichum nicotianae and Pythium aphanidermatum. SOD and POD activity was significantly enhanced, and MAD content was extremely reduced besides the transgenic tobacco seedlings had better growth traits. Therefore, PsnsLTP improved the ability of transgenic tobacco to resist biological and abiotic stress.

Key words: Polygonum sibiricum Laxm, non-specific lipid transfer protein, transgenic tobacco, biotic stress, abiotic stress

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