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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (3): 325-329.doi: 10.7525/j.issn.1673-5102.2013.03.012

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Enhanced Heavy Metal Tolerance and Accumulation of E.coli by Overexpressed AtGCS from Arabidopsis thaliana

LIU Da-Li;MAO Zi-Jun;AN Zhi-Gang*;MA Long-Biao;LU Zhen-Qiang   

  1. 1.Key Laboratory of Forest Plant Ecology,the Ministry of Education of China,Northeast Forestry University,Harbin 150040;2.Key Laboratory of Sugar beet Genetics and Breeding,Academy of Crop Sciences,Heilongjiang University,Harbin 150080;3.Key Laboratory of Biochemistry and Molecular Biology,College of Life Sciences,Heilongjiang University,Harbin 150080
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-05-20 Published:2013-05-20
  • Contact: AN Zhi-Gang
  • Supported by:

Abstract: GSH is one of the most ubiquitous proteins known to provide protection against toxic heavy metals. A gene-AtGCS encoding Arabidopsis γ-glutamylcysteine synthetase was introduced into E.coli (BL21) by over-expression of TrxA-AtGCS fusion protein to analyze the capacity to enhance heavy metal tolerance of transformed E.coli. As a comparison, strain over-expressing only TrxA was used as a control. The results showed that the growth of E.coli cells by over-expressing TrxA-AtGCS was much better than the control cells expressing TrxA under 1 mmol·L-1 Cd2+, Zn2+ and Cu2+ heavy metal stresses. Meanwhile, more than 5 times’ bioaccumulation of Cd2+, Zn2+, Cu2+ and more than 4 times’ GSH content were observed in the strain over-expressing TrxA-AtGCS relatively to control cells, especially under cadmium ions with 10 times’ accumulation in cells over-expressing TrxA-AtGCS. It could be concluded that over-expression of AtGCS offered a promising heavy metal resistance of E.coli with superior heavy metal accumulation and GSH content.

Key words: E.coli, heavy metal tolerance, AtGCS, GSH, accumulation

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