Abstract: This study used ISSR molecular marker technology to identify Larix gmelinii, Larix olgensis, Larix kaempferi and different clones. Thirteen ISSR primers which screened from fortynine primers can identify among larch species and clones，and the rate of the samples with specific band is 100%, this technology offers new ways and means for accurate identification of new larch species and elite seeds: there are five primers by which L. kaempferi, L. olgensis and L. kaempferi were amplified particularly, specific bands were produced at different location, as a species identification standards, nine primers can be amplified specific fragment in different kinds of larch clones respectively. It can identify the clones.

Key words: larch, species, clone, ISSR, identify