Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (2): 251-256.doi: 10.7525/j.issn.1673-5102.2020.02.012

• Research Report • Previous Articles     Next Articles

Function of Populus trichocarpa HDA902 Gene in Response to Cold Stress

GUAN Tao, LIU Chao, LI Kai-Long, XIA De-An, MA Xu-Jun   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-07-07 Online:2020-03-05 Published:2020-03-06
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572015CA11)

Abstract: Histone deacetylases plays an important role in plant abiotic stress responses. Histone deacetylase gene HDA902 was cloned from Populus trichocarpa by RT-PCR. The HDA902 gene was transformed into tobacco by Agrobacterium tumefaciens-mediated method. The tolerance of transgenic plants to cold stress was analyzed. The results showed that the expression of HDA902 in tobacco significantly improved the tolerance of transgenic lines to low temperature. The results of NBT and DAB staining showed that transgenic tobacco produced less reactive oxygen species than the wild type after cold treatment. The proline content in the leaves of transgenic plants were much higher than that of wild-type plants, whereas the MDA contents in the leaves of transgenic plants were much lower at low temperature. These results indicated that HDA902 was involved in the response to cold stress, and its overexpression enhanced the tolerance of transgenic plants to cold stress.

Key words: tobacco, HDA902, cold stress, tolerance

CLC Number: