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Bulletin of Botanical Research ›› 2012, Vol. 32 ›› Issue (3): 373-376.doi: 10.7525/j.issn.1673-5102.2012.03.022

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Optimization of Homogenate Extraction Technology of CoQ10 in Tobacco Leaves by Orthogonal Experiments

LI Chun-Ying;ZHAO Chun-Jian;ZHANG Bao-You;Liu Shuai-Hua;Liu De-Man;ZU Yuan-Gang   

  1. Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20
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Abstract: Using tobacco leaves as materials, the homogenate extraction technology of coenzyme Q10 (CoQ10) in tobacco was optimized by orthogonal experiments. The effect of homogenate time, ethanol volume percent, ratio of liquid to material and number of extraction cycles on the extraction yield of CoQ10 was investigated. The optimized homogenate extraction technology was determined as follows: 85% ethanol as extraction solvent, homogenate time 6 min,ratio of liquid to material 9∶1(mL· g-1), 2 extraction cycles. A comparison between homogenate and Soxhlet extractions was conducted. The extraction yield of CoQ10 was 12.82 μg·g-1 by homogenate extraction, which was almost equivalent to Soxhlet extraction. Homogenate extraction is faster and needs less solvent, thereby homogenate extraction has an advantage over Soxhlet extraction.

Key words: tobacco, CoQ10, homogenate extraction, orthogonal experiment

CLC Number: