Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (1): 132-140.doi: 10.7525/j.issn.1673-5102.2018.01.016

Previous Articles     Next Articles

Cloning and Expression Analysis of S-Adenosine Methionine Decarboxylase(ThSAMDC) Gene from Tamarix ramosissima

ZHANG Yu1, ZHANG Yue1, ZHANG Chun-Rui1, WANG Yan-Min1,2, WANG Yu-Cheng1, WANG Chao1   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
    2. Forestry Science Reseach Institute of Heilongjiang Province, Harbin 150081
  • Received:2017-07-04 Online:2018-01-15 Published:2018-01-06
  • Supported by:
    National Natural Science Foundation of Heilongjiang Province(QC2016019);Fundamental Research Funds for the Central Universities(2572014CA12)

Abstract: S-adenosyl-L-methionine decarboxylase(SAMDC) is a rate-limiting enzyme that regulates the physiological and biochemical processes of plants by participating in the metabolic pathway of polyamines. The cDNA of S-Adenosine Methionine Decarboxylase gene(named ThSAMDC) was isolated and cloned by analyzing the transcriptome data of Tamarix hispida. The full-length of ThSAMDC is 2 085 bp, with three open reading frames, tiny ORF(tORF), upstream ORF(uORF) and main ORF(mORF). The mORF was 1 107 bp encoding 369 amino acids. The relative molecular weight and isoelectric points(PI) of the putative protein were 40.34 kD and 4.72. ThSAMDC-encoded protein has a number of strong hydrophilic regions, no obvious transmembrane region. Compared to the amino acid multiple sequence alignment of several other species, ThSAMDC includes two highly conserved domains: the proenzyme cleavage site(LSESSLF) and the PEST domain(TIHVTPEDGFSYAS) associated with the rapid degradation of the protein. Phylogenetic tree analysis shows that ThSAMDC has higher sequence similarity of 77% identities to the Spinach(SoSAMDC). Quantitative real-time PCR assay revealed that the mRNA level of ThSAMDC was significantly up-regulated under NaCl, PEG, ABA and CdCl2 treatments in T.hispida, suggesting that ThSAMDC may play an important role in drought resistance, salt tolerance and other stresses.

Key words: Tamarix hispida, S-adenosylmethionine decarboxylase, stress responses, gene expression

CLC Number: