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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (5): 753-759.doi: 10.7525/j.issn.1673-5102.2016.05.017

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Cloning and Expression Analysis of CjCor1 Gene cDNA from Camellia japonica(Nai Dong)

YANG Kai1,2, SUN Ying-Kun2, WANG Kui-Ling2, LIU Qing-Hua2, GAO Han-Dong1   

  1. 1. Nanjing Forestry University, Nanjing 210037;
    2. Qingdao Agricultural University, Qingdao 266109
  • Received:2016-05-16 Online:2016-09-15 Published:2016-09-27
  • Supported by:
    Colleges and universities science & technology plan in Shandong province(No.J13LE16);National Science & Technology Pillar Program during the Twelfth Five-year Plan Period(No.2012BAD01B0703)

Abstract: COR genes play important roles in plant cold acclimation process. In order to study the Cor gene function of Camellia japonica to severe cold, adopting homologous cloning method, a 617 bp full-length cDNA sequence was cloned from leaves of C.japonica(Nai Dong) under the natural low temperature condition, named CjCor1. The total length sequence is complete, and contains an opening reading frame of 600 bp. Amino acids sequence alignment shows that CjCor1 gene encodes a protein of 199 amino acids, containing specific conservative domain structure of WCOR413 in cold acclimatization protein superfamily, and highly conservative property in species belonged to same genus. By real-time PCR analysis, comparing with southern Camellia species and common varieties, CjCor1 genes had higher expression in C.japonica(Nai Dong) under the condition of low temperature(below 0℃), and also had higher expression in higher cold resistance varieties.

Key words: Camellia japonica(Nai Dong), CjCor1 gene, sequence analysis, gene expression

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