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植物研究 ›› 2019, Vol. 39 ›› Issue (1): 104-112.doi: 10.7525/j.issn.1673-5102.2019.01.013

• 研究报告 • 上一篇    下一篇

干旱胁迫下拟南芥中H2S与ABA信号关系研究

赵敏1, 王玥萱1, 徐运飞2, 赵启安1, 刘博1, 杨宁1   

  1. 1. 西北师范大学生命科学学院, 兰州 730070;
    2. 信阳农林学院农学院, 信阳 464000
  • 收稿日期:2018-09-04 出版日期:2019-01-15 发布日期:2019-01-31
  • 通讯作者: 杨宁 E-mail:xbsd-yn@163.com
  • 作者简介:赵敏(1993-),女,硕士研究生,主要从事植物细胞信号转导方面的研究。
  • 基金资助:
    国家自然科学基金(31660116)

Relationship between H2S and ABA Signaling in Arabidopsis thaliana under Drought Stress

ZHAO Min1, WANG Yue-Xuan1, XU Yun-Fei2, ZHAO Qi-An1, LIU Bo1, YANG Ning1   

  1. 1. College of Life Science, Northwest Normal University, Lanzhou 730070;
    2. Xinyang Agriculture and Forestry University, Xinyang 464000
  • Received:2018-09-04 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    National Natural Science Foundation of China(31660116)

摘要: 以野生型拟南芥(WT)、硫化氢(H2S)合成酶缺失型突变体lcd、脱落酸(ABA)合成缺失型突变体aba1实生苗为材料,以0.3 mol·L-1甘露醇模拟干旱胁迫,研究干旱胁迫对ABA含量、H2S含量的影响,及其在拟南芥抵抗干旱胁迫中的作用及信号关系。结果显示:干旱胁迫显著提高LCDABA1基因相对表达以及H2S含量,ABA含量;干旱胁迫显著抑制突变体lcd、aba1的种子萌发;干旱胁迫下,外施NaHS促进干旱胁迫下WT、lcdaba1中內源H2S的产生及上调LCD、ABA1基因相对表达,而外施ABA提高干旱胁迫下WT、aba1中H2S含量及LCD、ABA1基因相对表达,但是对lcd中H2S含量及LCD基因相对表达没有显著影响。研究结果表明,信号分子H2S和ABA在拟南芥的干旱胁迫响应中发挥一定的作用,且H2S位于ABA的下游参与调控拟南芥的信号过程。

关键词: 拟南芥, 干旱胁迫, 硫化氢, 脱落酸

Abstract: With the seedlings of wild type Arabidopsis thaliana(WT), hydrogen sulfide(H2S) synthase deletion mutant lcd, abscisic acid(ABA) deletion mutant aba1, 0.3 mol·L-1 mannitol simulated drought stress, we studied the effects of drought stress on ABA content and H2S content, analyzed their roles and signal relationships in drought tolerance. Drought stress significantly increased the LCD and ABA1 gene relative expressions, contents of H2S and ABA; Drought stress significantly inhibited the seed germination of lcd and aba1 mutants; Application of NaHS and ABA significantly increased the endogenous H2S content in WT under drought stress; Application of NaHS could promote the endogenous H2S production in WT, lcd and aba1, the relative expression of LCD and ABA1 also up-regulated under drought stress. While the application of ABA increased the H2S content and the LCD, ABA1 relative expressions in WT and aba1 under drought stress, but had no significant effect on the H2S content and LCD relative expression in lcd. The results showed that the signal molecules H2S and ABA played a vital role response to the drought stress in Arabidopsis, and H2S is located at downstream of ABA to participated in the regulation of signaling process.

Key words: Arabidopsis thaliana, drought stress, hydrogen sulfide, abscisic acid

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