过表达PtrMYB002基因抑制拟南芥生长并提高其抗旱性

doi:10.7525/j.issn.1673-5102.2026.01.006

摘要/Abstract

摘要：

该研究针对全球干旱加剧导致杨树生长受抑及死亡的问题，以毛果杨（Populus trichocarpa）R2R3-MYB转录因子PtrMYB002为对象，通过基因克隆、表达载体构建、拟南芥（Arabidopsis thaliana）遗传转化和杨树（Populus L.）瞬时转化等手段，结合生物信息学、亚细胞定位、表型观察、生理指标测定及基因表达分析等方法，探究其在植物生长与抗旱性调控中的功能。结果表明：PtrMYB002定位于细胞核，其表达受干旱与ABA处理的显著诱导；过表达PtrMYB002基因显著负影响拟南芥的子叶面积、下胚轴长度、根长、莲座直径、地上部分鲜质量及花薹高度，但提高其抗旱性，表现为干旱条件下转基因株系的净CO?同化速率、电子传递速率（ETR）、实际光化学效率（Y（Ⅱ））、光化学淬灭系数（qP）和非光化学淬灭系数（NPQ）均显著高于野生型（WT），而气孔导度、蒸腾速率和相对电导率则显著低于WT，同时转基因株系中抗旱相关基因AtRD29A与AtDREB2的相对表达量显著上调。此外，瞬时转化PtrMYB002基因的‘84K’杨（P. alba×P. glandulosa ‘84K’）中PagRD29A与PagDREB2B基因相对表达量显著高于对照组，表明其调控机制在杨树中可能具有一定的保守性。综上，过表达PtrMYB002基因抑制拟南芥生长，同时增强其抗旱性，体现了其参与植物“生长与防御权衡”策略，该研究结果为杨树抗旱分子育种提供了新的基因资源与理论依据。

关键词: 毛果杨, 拟南芥, PtrMYB002, 生长发育, 抗旱性

Abstract:

This study addressed the issue of growth suppression and mortality in poplar caused by increasing global drought， focusing on the R2R3-MYB transcription factor PtrMYB002 from Populus trichocarpa. Utilizing methods including gene cloning， expression vector construction， genetic transformation of Arabidopsis thaliana， and transient transformation in poplar， combined with bioinformatics analysis， subcellular localization， phenotypic observation， physiological index measurement， and gene expression analysis， we investigated its role in regulating plant growth and drought tolerance. The results showed that PtrMYB002 was localized in the nucleus and its expression was significantly induced by drought and ABA treatment. Overexpression of PtrMYB002 gene significantly inhibited the cotyledon area， hypocotyl length， root length， rosette diameter， above-ground fresh mass， and inflorescenced stem height in Arabidopsis， but enhanced its drought tolerance. Under drought conditions， the transgenic lines exhibited significantly higher net CO? assimilation rate， electron transport rate（ETR）， actual photochemical efficiency（Y（Ⅱ））， photochemical quenching coefficient（qP）， and non-photochemical quenching coefficient（NPQ）， while the stomatal conductance， transpiration rate， and relative electrical conductivity were significantly lower compared to the wild-type（WT）. Concurrently， the expression levels of drought-responsive genes AtRD29A and AtDREB2 were significantly upregulated in the transgenic lines. Furthermore， in the ‘84K’ poplar with transient overexpression of PtrMYB002 gene， the expression levels of PagRD29A and PagDREB2B genes were significantly higher than that in the control group， indicating that this regulatory mechanism was likely conserved in poplar. In conclusion， the overexpression of PtrMYB002 gene suppressed growth in Arabidopsis while enhancing its drought tolerance， demonstrating a potential role of the gene in participation of “growth and defense trade-off” strategy， and provided new genetic resources and a theoretical basis for molecular breeding of drought-tolerant poplar.

Key words: Populus trichocarpa, Arabidopsis thaliana, PtrMYB002, growth and development, drought resistance

中图分类号:

Q344

李政, 陈凤欣, 刘雨奇, 李明明, 尹嘉璨, 刘超, 夏新莉. 过表达PtrMYB002基因抑制拟南芥生长并提高其抗旱性[J]. 植物研究, 2026, 46(1): 67-82.

Zheng LI, Fengxin CHEN, Yuqi LIU, Mingming LI, Jiacan YIN, Chao LIU, Xinli XIA. Overexpression of the PtrMYB002 gene Inhibits Growth of Arabidopsis thaliana and Enhances Its Drought Resistance[J]. Bulletin of Botanical Research, 2026, 46(1): 67-82.

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引物名称 Primer name	上游引物序列（5′→3′） Forward primer sequence（5′→3′）	引物名称 Primer name	下游引物序列（5′→3′） Reverse primer sequence（5′→3′）
AtRD29A-F	CGGGATTTGACGGAGAACCA	AtRD29A-R	GGTCTCTTCCCAGCTCAGTC
AtDREB2-F	CGAGCAGCCGAAGAAAAGGA	AtDREB2-R	GCAGGAACTTTGCGTTTCGG
PagRD29A-F	GCCACAAATGTCATGGCCTC	PagRD29A-R	TCGCAGCATTTTGTCCTTGTC
PagDREB2B-F	GTTCTGCGCTTGGGTTTAGC	PagDREB2B-R	CTTTCTTCGACCCCTTGCCT
PtrMYB002-F	CGTTAGCAACCTCCATGAAAGC	PtrMYB002-R	TCACCACTGTTAAGGACTCCAAC
AtActin2-F	AGTGGTCGTACAACCGGTATTGT	AtActin2-R	GATGGCATGAGGAAGAGAGAAAC
PagUBQ-F	AGACCTACACCAAGCCCAAGAAGAT	PagUBQ-R	CCAGCACCGCACTCAGCATTAG