拟南芥中H2S与PLDα1响应干旱胁迫的作用研究

doi:10.7525/j.issn.1673-5102.2018.03.013

摘要/Abstract

摘要： 以野生型拟南芥WT、PLDα1合成缺陷突变体pldα1-1以及L-半胱氨酸脱巯基酶（L-cysteine desulfyrase，L-CDes）合成缺陷突变体lcd-4幼苗为材料，以0.3 mol·L^-1甘露醇模拟干旱胁迫，研究磷脂酶Dα1（phospholipase Dα1，PLDα1）与气体信号分子硫化氢（hydrogen sulfide，H₂S）响应干旱胁迫的作用及可能存在的信号关系。结果显示：干旱胁迫下，野生型拟南芥的H₂S含量、PLD与L/D-CDes活性及其基因相对表达量均发生显著的变化。萌发率实验中，与WT相比，pldα1-1和lcd-4对干旱胁迫更加敏感，外施NaHS可以促进干旱胁迫下WT、pldα1-1以及lcd-4种子萌发及內源H₂S产生，而外施PA能提高干旱胁迫下WT、pldα1-1种子萌发率及H₂S含量，但对lcd-4萌发率及H₂S含量没有显著影响。研究结果表明，信号分子H₂S和PLDα1在拟南芥的干旱胁迫响应中发挥一定的作用，且H₂S可能位于PLDα1的下游参与调控拟南芥种子萌发的信号过程。

关键词: 拟南芥, 干旱胁迫, 硫化氢, 磷脂酶Dα1

Abstract: The experiment was conducted to study the role of phospholipase Dα1(PLDα1) and the gas signal hydrogen sulfide(H₂S) in response to drought stress and the possible signal relationship. The wild type Arabidopsis thaliana(WT), PLDα1 mutant(pldα1-1) and the LCD mutant plant(lcd-4) was used as experiment materials, and 0.3 mol·L^-1 mannitol was used to simulate drought stress. The content of endogenous H₂S, the activities of PLD, L/D-CDes, and the relative expression of PLDα1, LCD and DCD in wild type Arabidopsis were significantly changed under drought stress. Compared with WT, pldα1-1 and lcd-4 were more sensitive to drought condition. Application of NaHS could promote the seed germination and endogenous H₂S production in WT, pldα1-1 and lcd-4. Exogenous addition of PA could increase the germination rate and endogenous H₂S concentration in WT and pldα1-1, but had no significant effect in lcd-4. The signal molecules H₂S and PLDα1 played important roles in the plant response to drought stress, and H₂S might be located at downstream of PLDα1 in signal transduction process to regulate the seed germination of A. thaliana.

Key words: Arabidopsis thaliana, drought stress, hydrogen sulfide, phospholipase Dα1

中图分类号:

Q949.748.3

赵敏, 杨宁, 陈璐, 安炎黄, 李文领, 赵峰峰. 拟南芥中H₂S与PLDα1响应干旱胁迫的作用研究[J]. 植物研究, 2018, 38(3): 406-414.

ZHAO Min, YANG Ning, CHEN Lu, AN Yan-Huang, LI Wen-Ling, ZHAO Feng-Feng. Effects of H₂S and PLDα1 on Drought Stress in Arabidopsis thaliana[J]. Bulletin of Botanical Research, 2018, 38(3): 406-414.

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拟南芥中H₂S与PLDα1响应干旱胁迫的作用研究

Effects of H₂S and PLDα1 on Drought Stress in Arabidopsis thaliana

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