刚毛柽柳S-腺苷甲硫氨酸合成酶(ThSAMS)基因的克隆及表达分析

doi:10.7525/j.issn.1673-5102.2019.01.014

摘要/Abstract

摘要： 通过对刚毛柽柳转录组分析，克隆获得了一条与S-腺苷甲硫氨酸合成酶（SAMS）基因同源性高的基因，命名为ThSAMS。序列分析结果表明：ThSASM基因全长cDNA为1 185 bp，编码394个氨基酸，编码蛋白相对分子质量为97.85 kDa，理论等电点为5.02。通过生物信息学分析表明，ThSASM基因编码的氨基酸与其他物种SAMS基因编码的氨基酸具有很高的同源性，其中与枣的同源性最高，达95%。实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）分析表明，ThSASM表达受NaCl、聚乙二醇（PEG）和ABA处理做出应答，暗示ThSASM可能参与了刚毛柽柳对盐和干旱的胁迫应答，为进一步研究SAMS基因在植物胁迫应答中的功能及作用机制提供了参考依据。

关键词: 刚毛柽柳, ThSAMS基因, S-腺苷甲硫氨酸脱羧酶, 基因表达

Abstract: A full length cDNA of a S-Adenosyl-L-Methionine Synthetase gene(named ThSAMS) was isolated from the transcriptome cDNA librarys of Tamarix hispida. The open reading frame of ThSAMS cDNA was 1 185 bp encoding 394 amino acids. The relative molecular weight of ThSAMS protein was 97.85 kDa, with isoelectric point of 5.02. Multiple alignment analysis based on the amino acids indicated that ThSAMS had high homology to SAMS proteins from different species, and the highest similarity of 95% to the Ziziphus jujuba SAMS. Quantitative real-time PCR(qRT-PCR) assay revealed that ThSAMS gene can respond to NaCl, PEG and ABA stress, suggesting that it may be involved in salt and drought stresses in T.hispida. This study provided theoretical groundwork for further exploration into the function of stress tolerance of SAMS gene in plants.

Key words: Tamarix hispida, ThSAMS gene, S-Adenosyl-L-Methionine Synthetase, gene expression

中图分类号:

S793.5

张悦, 赵鑫, 侯峥, 王艳敏, 王玉成, 王超. 刚毛柽柳S-腺苷甲硫氨酸合成酶(ThSAMS)基因的克隆及表达分析[J]. 植物研究, 2019, 39(1): 113-122.

ZHANG Yue, ZHAO Xin, HOU Zheng, WANG Yan-Min, WANG Yu-Cheng, WANG Chao. Cloning and Expression Analysis of S-Adenosyl-L-Methionine Synthetase(ThSAMS) Gene from Tamarix hispida[J]. Bulletin of Botanical Research, 2019, 39(1): 113-122.

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