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植物研究 ›› 2021, Vol. 41 ›› Issue (4): 531-539.doi: 10.7525/j.issn.1673-5102.2021.04.008

• 研究报告 • 上一篇    

巴西橡胶树赤霉素信号转导途径HbRGA1结构和功能分析

陈华峰1, 樊松乐2, 王立丰2()   

  1. 1.东北林业大学化学化工与资源利用学院,森林植物生态学教育部重点实验室,哈尔滨 150040
    2.农业农村部橡胶树生物学与遗传资源利用重点实验室,省部共建国家重点实验室培育基地—海南省热带作物栽培生理学重点实验室,农业农村部儋州热带作物科学观测实验站,中国热带农业科学院橡胶研究所,海口 571101
  • 收稿日期:2020-12-15 出版日期:2021-07-20 发布日期:2021-03-24
  • 通讯作者: 王立丰 E-mail:lfwang@catas.cn
  • 作者简介:陈华峰(1978—),男,硕士,主要从事植物代谢及生理生态学研究。
  • 基金资助:
    国家重点研发专项(2020YFD1001203)

Structural and Functional Analysis of Gibberellin Signaling Transduction Pathway Gene HbRGA1 in Rubber Tree

Hua-Feng CHEN1, Song-Le FAN2, Li-Feng WANG2()   

  1. 1.College of Chemistry,Chemical Engineering and Resource Utilization,Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040
    2.Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,P. R. China;State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops;Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,P. R. China;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
  • Received:2020-12-15 Online:2021-07-20 Published:2021-03-24
  • Contact: Li-Feng WANG E-mail:lfwang@catas.cn
  • About author:CHEN Hua-Feng(1978—),male,postgraduate,major in plant Metabolism and Physiological Ecology.
  • Supported by:
    National Key Research and Development Project(2020YFD1001203)

摘要:

DELLA蛋白是赤霉素激素信号负调控因子,具有抑制植物生长发育的作用。解析其家族成员结构与功能将有助于揭示橡胶树DELLA蛋白家族成员调控橡胶树生长发育的机制。本研究从橡胶树热研73397叶片中克隆HbRGA1的cDNA全长序列。该基因长为2 136 bp,含1 839 bp的ORF,编码613个氨基酸。HbRGA1蛋白序列包含DELLA和GRAS保守结构域,与杨树、木薯和橡胶树DELLA基因相似性高达82.5%。qRT-PCR分析发现HbRGA1在橡胶树叶片中表达量高,在树皮和胶乳中表达量极低。叶片中HbRGA1表达量受喷施赤霉素和脱落酸等诱导显著上调。本研究表明HbRGA1与橡胶树赤霉素等激素信号密切相关,为深入研究其在橡胶树生长发育中的结构和功能打下良好基础。

关键词: 巴西橡胶树, HbRGA1, 赤霉素, 基因表达

Abstract:

DELLA protein is a negative regulator of gibberellin signaling pathway, which inhibits plant growth and development. Analyzing the structure and function of DELLA family members will be helpful to elucidate the mechanism by which the DELLA protein regulates the growth and development of rubber trees. In this study, the full-length cDNA sequence of HbRGA1 was cloned from the leaves of rubber tree cultivar Reyan73397. HbRGA1 is 2 136 bp long, contains 1 839 bp ORF, and encodes 613 amino acids. The HbRGA1 protein sequence contains DELLA and GRAS superfamily conserved domains, which are 82.5% like the DELLA genes of poplar, cassava and rubber trees. qRT-PCR analysis showed that the expression level of HbRGA1 was high in the leaf of rubber tree, but very low in the bark and latex. The expression of HbRGA1 in leaves was significantly up-regulated by gibberellin and abscisic acid spraying. These results indicated that HbRGA1 was closely related to gibberellin and other hormone signals in rubber trees, which laid a good foundation for further study of HbRGA1’s structure and function in the growth and development of rubber trees.

Key words: Hevea brasiliensis, HbRGA1, gibberellin, gene expression

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