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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (5): 704-713.doi: 10.7525/j.issn.1673-5102.2018.05.010

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Multiple Roles of Berberine Bridge Enzyme Gene HbBBE1 in Response to Stress in Hevea brasiliensis Muell. Arg.

WANG Meng, ZHNG Dong, LU Yan-Xi, XIAO Hua-Xing, ZHENG Fu-Cong, ZHANG Yu   

  1. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228
  • Received:2018-01-21 Online:2018-09-15 Published:2018-08-31
  • Supported by:
    the Earmarked Fund for Modern Agro-industry Technology Research System(CARS-33-BC1);National Natural Science Foundation of China(31660204,31660187);Doctoral research foundation of Hainan University(KYQD(ZR)1733)

Abstract: Berberine bridge enzyme(BBE,EC1.5.3.9) is a key enzyme in berberine biosynthesis. In this study, HbBBE1 gene was cloned in rubber tree by RT-PCR. The HbBBE1 promoter contained light, gibberellin, salicylic acid, wounding, fungal elicitor, defense, and stress responsive elements. The predicted protein of HbBBE1 contains a signal peptide, a trans membrane region, an FAD binding domain, and a BBE domain which is found specifically in the berberine bridge enzymes and berberine bridge-like enzymes. It shared the highest similarity with BBE protein from Manihot esculenta with 80.1% identical residues. Comparing expression among different tissues of rubber tree demonstrated that HbBBE1 has a particularly high expression level in the latex. HbBBE1 expression was significantly upregulated by biotic and abiotic stress, i.e. powdery mildew infection, drought stress, high intensity light irradiation, H2O2, and both tapping treatment in virgin trees and mechanical wounding in seedlings. Furthermore, HbBBE1 transcript was remarkably induced by application of exogenous hormones, including gibberellic acid(GA3), salicylic acid(SA), ethephon(ET), methyl jasmonate(MeJA), and abscisic acid(ABA). Among these treatments, HbBBE1 transcript was quickly induced by GA3 stimulation, and reached a maximum(6.3-fold) at 0.5 h. The increased expression of HbBBE1 was highly induced by ABA, ET, and H2O2 stimuli, and it accumulated dozens of times comparing that at the untreated control.To summarize, our study suggested that HbBBE1 played multiple roles in response to biotic and abiotic stress in rubber tree.

Key words: Hevea brasiliensis, berberine bridge enzyme, HbBBE1, stress response

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