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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (4): 575-582.doi: 10.7525/j.issn.1673-5102.2020.04.012

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Gene Cloning and Stress Response Analysis of Natural Disorder Protein in Populus trichocarpa

DONG Shi-Wei, YANG Yu-Ning, WANG Nai-Rui, ZHANG Han-Guo, LI Shu-Juan   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-12-29 Online:2020-07-05 Published:2020-06-12
  • Supported by:
    Heilongjiang Science Fund Project(C2017008)

Abstract: In order to know the related information of PtrIDP1(Potri.010G161200.1) gene in Populus trichocarpa and to explore the expression characteristics of the gene in different tissues and under different stress, primers were designed according to the full-length sequence of the gene obtained from Phytozome database, and the target fragment of the gene was cloned. The complete CDs region of the gene is 423 bp long and encodes 140 amino acids. The expression vector of subcellular localization was constructed, and the epidermal cells of the onion were transiently transformed. The result showed that the PtrIDP1 was located in the cell nucleus under the laser confocal microscope. The expression specificity of PtrIDP1 gene in different tissues of P.trichocarpa and its response to abiotic stress were analyzed by real-time quantitative RT-PCR. The results showed that PtrIDP1 gene was expressed in the roots, stems and leaves of P.trichocarpa, but the lowest expression was in the roots, whereas the relative expression was higher in the stems and leaves. The expression patterns of PtrIDP1 gene were different under the induction of high salt and drought stress, and the preliminary analysis suggested that PtrIDP1 gene was involved in the process of responding to abiotic stress in P.trichocarpa.

Key words: Populus trichocarpa, gene cloning, stress response to adversity, intrinsically disordered proteins

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