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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (1): 91-99.doi: 10.7525/j.issn.1673-5102.2018.01.011

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Transcriptomic Analysis of the Halostachys caspica in Response to Short-term Salt Stress

ZHANG Li-Li1,2, ZHANG Fu-Chun1,2   

  1. 1. College of Life Science and Technology, Xinjiang University, Urumqi 830046;
    2. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046
  • Received:2017-05-08 Online:2018-01-15 Published:2018-01-06
  • Supported by:
    Supported by Special Funds of Xinjiang Key Laboratory(2014KL001)

Abstract: As a halophyte with strong salt tolerance, Halostachys caspica widely distributes in desert saline-alkali land. In order to reveal the genomic changes of gene expression under salt stress, transcriptome sequencing of H.caspica assimilating branches with treatments of 300 and 500 mmol·L-1 NaCl for 3 h was performed. A total of 153 298 unigenes with an average length of 643 bp were obtained with clean reads assembled. The 47 subclasses and 118 KEGG pathways were enriched in the GO terms and KEGG pathways, respectively. Differentially expressed genes analysis showed that there were 4 432 and 2 580 unigenes in response to low salt (300 mmol·L-1) and high salt (500 mmol·L-1) in short-term stress, respectively. The 1 245 unigenes were the common differentially expressed genes in the two salt stresses. They were mainly enriched in cellular process, metabolic process and response to stimulus. The osmotic regulation and reactive oxygen species (ROS) scavenging genes were screened out, and most of them were up-regulated. Therefore, H.caspica could improve short-term salt stress adaptation by enhancing the osmotic adjustment and ROS scavenging.

Key words: Halostachys caspica, salt stress, transcriptome, differentially expressed genes, osmotic adjustment, reactive oxygen species

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