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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (6): 909-916.doi: 10.7525/j.issn.1673-5102.2016.06.015

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Bioinformatics and Expression Analysis of BpCesA Genes of Betula platyphylla

SHEN Ting-Ting1, JIANG Jing1, LIU Gui-Feng1, XU Si-Jia1, LI Hui-Yu1, YUAN Hong-Mei1,2   

  1. 1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
    2. Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086
  • Received:2016-09-09 Online:2016-11-15 Published:2016-11-16
  • Supported by:
    National Science and Technology Planning Project of "12th Five-Year"(2012BAD21B02); State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University(201203)

Abstract: Six cellulose synthase genes of Betula platyphylla from 45 transcriptome data sets were obtained, in which two genes were new genes of cellulose synthase gene family. The sequence characteristic and expression levels in Birch xylem of BpCesA were analyzed using bioinformatics and quantitative real-time PCR. The ORF length of 6 BpCesA genes varied from 2 958-3 312 bp; Number of amino acids encoded varied from 985-1 103 aa, and relative molecular weight varied from 110.40-124.29 kD; Theoretical isoelectric point was from 5.90-7.43. Six BpCesA genes all contained D, D, D, QXXRW conservative structure domain and eight transmembrane helices, in which two were located at the end of the N, and the others were at the end of the C. 52 CesAs from six dicotyledonous plants, four monocotyledons, and one gymnosperms were analyzed by cluster analysis, and BpCesAs had higher homology with PtrCesA. The homology between BpCesA2 and PtrCesA3 reached 92%. BpCesA5 and PtrCesA6, BpCesA1 and PtrCesA8 also had high homology of 89% and 82%, respectively. The RNA level of BpCesA1, BpCesA2, BpCesA3 and BpCesA5 was increased with the increase of cellulose content. This results suggested that these genes play a positive role in cellulose synthesis in birch. This study provides a basis for the functional analysis of cellulose synthase gene.

Key words: Betula platyphylla, cellulose, lignin, bioinformatics, expression quantity

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