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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 191-199.doi: 10.7525/j.issn.1673-5102.2022.02.004

• Genetic and Breeding • Previous Articles     Next Articles

Variation of Lignin Content and Association Analysis of FmPAL Nucleotide Polymorphism in Progenies of Interspecific Hybrids of Fraxinus

Yibo Yin1, Jixiang Li1, Yingjie Guo1, Ziting Lu1, Ying Xiao2, Hualing Liu2, Yaguang Zhan1, Fansuo Zeng1()   

  1. 1.College of Life Sciences,Northeast Forestry University,Harbin 150040
    2.Shanhetun Forestry Bureau of Heilongjiang Province,Harbin 150232
  • Received:2021-05-19 Online:2022-03-20 Published:2022-02-22
  • Contact: Fansuo Zeng E-mail:zengfansuo@126.com
  • About author:Yin Yibo(1996—),female,master,majoring in the research of plant genetic engineering.
  • Supported by:
    Applied Technology Research and Development Program of Heilongjiang Province(GA19B201);Innovative Training Program for College Students(201910225169)


To improve materials for genetic breeding of Fraxinus mandshurica, the correlation among three hybrid combinations of Fraxinus mandshurica × F. rhynchophyllaF. mandshurica × F. sogdiana and F. mandshurica × F. velutina, and the genetic variation of lignin content and FmPAL nucleotide polymorphism in free-pollination offspring of female parent were analyzed respectively. One-year-old branches of Fraxinus mandshurica with 71 species of interspecies hybrid combinations in Maoer Mountain Forest Farm of Northeast Forestry University were used as materials, the genetic variation regularity of lignin was investigated, the differences in lignin content and variation parameters and mean values were analyzed, and joint analysis on the molecular level of lignin and its synthesis-related gene PAL were carried out. The result showed that the lignin content of the hybrid combinations of Fraxinus was extremely significant, and it was significantly different from the female parent free-pollinated offspring of F. mandshurica. The lignin content of most hybrid combinations of F. mandshurica was higher than that of the female parent F. mandshurica. The results showed that the haploid diversity value of the PAL gene of the F. mandshurica population reached 0.999 7, which was a high level. It confirmed that the FmPAL gene had a high variation. There were 3 SNP sites significantly related to the lignin content. There were significant differences in the lignin content of the cross combinations of F. mandshurica. The SNP sites in the FmPAL gene was significantly related to the lignin content.

Key words: Fraxinus, Fraxinus mandshurica, Lignin, Genetic variation, FmPAL, SNP

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