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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (3): 325-328.doi: 10.7525/j.issn.1673-5102.2009.03.014

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Study on the Germination and Plant Regeneration of Lolium perenne

ZHOU Xiao-Mei;XU Jin;LI Jun-Jian*   

  1. (1.Life Science and Technology College,Shanxi University,Taiyuan030006) (2.Institute of Loess Plateau,Shanxi University,Taiyuan030006)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: LI Jun-Jian
  • Supported by:

Abstract: We studied the effects of pre-treatments on seed germination, callus inducement and the plant regeneration of Lolium perenne. The results showed that pre-treatments including seeds drying 35℃ for 3 h, immersion for 16 hours then drying for 48 h and irradiation under 1 000 Gs magnetic field promoted the germination ratios significantly. MS medium was the optimal one in the three media tested. The 6 mg·L-1 2,4-D was suitable for inducing callus formation from seeds. The medium for callus differentiation into plantlets contained 2 mg·L-1 cytokinin (6-BA or KT). The basic MS medium adding 0.5 mg·L-1 NAA was best for regenerated plantlets rooting.

Key words: Lolium perenne, seeds, pre-treatments, callus, plant regeneration

CLC Number: